Construct: ORF ccsbBroad304_13178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002122.1_s304c1, BRDN0000411677
- Derived from:
- ccsbBroadEn_13178
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JMY (133746)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13178
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133746 | JMY | junction mediating and regu... | NM_152405.5 | 62.9% | 62.9% | 1_1062del;2346C>T;2429_2464del |
| 2 | human | 133746 | JMY | junction mediating and regu... | XM_011543155.3 | 59.6% | 59.7% | (many diffs) |
| 3 | human | 133746 | JMY | junction mediating and regu... | XM_005248430.3 | 58.7% | 58.8% | (many diffs) |
| 4 | mouse | 57748 | Jmy | junction-mediating and regu... | NM_021310.3 | 55.7% | 56.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1935
- ORF length:
- 1866
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggagctt ctggacttgt atcagatgga ggatgaagcc tacagcagcc 121 ttgcagaagc tacaaccgaa ctctatcagt atttactaca gccattccga gacatgagag 181 aacttgccat gctacgaaga cagcagatca agatttccat ggagaatgat tatctgggac 241 ctcgaagaat tgagagtcta caaaaagaag atgctgattg gcagcggaaa gctcacatgg 301 ctgtactgtc tattcaagat cttactgtca agtactttga aataacagct aaagctcaaa 361 aagctgtgta tgatcgaatg cgagctgatc agaagaaatt tggtaaagca tcatgggcag 421 cggctgctga acggatggaa aaactccagt atgcagtttc taaggaaact ttgcagatga 481 tgagagctaa agagatatgc ttggaacagc ggaaacatgc actaaaggaa gagatgcaga 541 gtttgcgggg tggtacagaa gcgatagcac gattggatca gttagaagct gattattatg 601 atctgcaact tcagttgtat gaagtacagt ttgaaatctt gaagtgtgaa gagttactat 661 tgacagcgca actagaaagc atcaaaagac ttatatcaga aaaaagagat gaagtggtat 721 actatgacac ttacgaaagc atggaggcca tgctggagaa ggaagagatg gcagcatctg 781 cgtacttaca gagagaagag ctgcagaaac ttcagcagaa agcacgccag ctggaagcaa 841 gacgtggacg ggtttctgcc aagaaatcct acctcagaaa taaaaaggaa atatgtattg 901 caaaacacaa tgaaaaaatc caacagcgca ctcggattga agatgaatat agaacccatc 961 acacagtaca actaaagaga gaaaaattac atgatgaaga agaaagaaaa agtgcctggg 1021 ttagccaaga gagacagaga acactggata gacttcgaac atttaaacag aggtatcctg 1081 ggcaagtcat acttaaatca accagattac gactagctca tgcaagaaga aaaggtgcag 1141 caagtcctgt tctccaagag gatcattgtg actctttacc aagtgtgtta caggtagaag 1201 agaaaactga agaggtggga gaaggaagag tcaagcgtgg gccatcacag acaacagaac 1261 cccagagcct tgtgcaactt gaagatactt cattaacaca acttgaagcc acctcattac 1321 ctctcagtgg tgttacctct gaactgcctc ctactatatc tcttccactt ttgaataaca 1381 acctcgaacc atgttctgtt accataaatc cactcccatc ccctcttcct ccaacaccac 1441 cacctctgcc tgttgctaag gacagtggcc cagagacact ggagaaagat ctgcctagaa 1501 aggaggggaa tgagaagagg atcccaaagt cagccagtgc cccctcagca cacctctttg 1561 acagcagcca gctggtcagt gcacggaaga agctcagaaa gactgctgaa ggtttgcaga 1621 ggaggagagt gagctcaccc atggatgagg tgctagcctc cttgaagcgt ggtagttttc 1681 atctgaaaaa ggttgaacag cgaactctgc ctccttttcc tgatgaagat gatagtaata 1741 atatcttggc acaaataagg aaaggggtaa aattgaagaa ggtacagaag gatgttttga 1801 gagaatcctt cacacttcta cccgatacag accctctaac acggagcatc catgaagctc 1861 ttagaagaat taaagaagca tccccagagt cagaggacga agaggaggct ttaccttgca 1921 cagactggga gaacttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 1981 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2041 cgacaatcaa cctctggatt a