Construct: ORF ccsbBroad304_13183

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004102.1_s304c1, BRDN0000408475
Derived from:
ccsbBroadEn_13183
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
B3GAT2 (135152)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13183
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 135152 B3GAT2 beta-1,3-glucuronyltransfer... NM_080742.3 77.7% 77.3% 374_589del
2 mouse 280645 B3gat2 beta-1,3-glucuronyltransfer... NM_172124.2 68.6% 69.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
822
ORF length:
753
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaagtccgcg cttttcaccc gcttctttat cctcctgccc tggatcctaa
121ttgtcatcat catgctcgac gtggacacgc gcaggccagt gcccccgctc accccgcgcc
181cctacttctc tccctacgcg gtgggccgcg ggggcgcccg actcccgctc cgcaggggcg
241gcccggctca cgggacccaa aagcgcaacc agtctcggcc gcagccacag ccggagccgc
301agctgcccac catctatgcc atcacgccca cctacagccg cccggtgcag aaagcggagc
361tgacccgcct ggccaacacg ttccgccagg tggcgcagct gcactggatc ctggtggagg
421acgcggcggc gcgcagcgag cagatgcgaa ccacccgcaa ggtctccgtc tggcctgtgg
481gcctggttgg tgggcggcgc tacgaacgtc cgctggtgga aaacggcaaa gttgttggct
541ggtacaccgg ctggagagca gacaggcctt ttgccatcga catggcagga tttgctgtaa
601gtcttcaagt cattttgtcc aatccaaaag ctgtatttaa gcgtcgtgga tcccagccag
661ggatgcaaga atctgacttt ctcaaacaga taacaacagt cgaagaactg gaaccgaaag
721caaataactg cactaaggtt ctcgtgtggc acactcggac agagaaggtt aatctagcca
781acgagccaaa gtaccacctg gacacagtga aaattgaggt attgccaact ttcttgtaca
841aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
901ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)