Construct: ORF ccsbBroad304_13212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009100.1_s304c1, BRDN0000408463
- Derived from:
- ccsbBroadEn_13212
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB14 (142686)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13212
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 142686 | ASB14 | ankyrin repeat and SOCS box... | NM_130387.5 | 71.3% | 64.3% | (many diffs) |
| 2 | human | 142686 | ASB14 | ankyrin repeat and SOCS box... | NM_001142733.3 | 35.6% | 31.5% | (many diffs) |
| 3 | human | 142686 | ASB14 | ankyrin repeat and SOCS box... | XM_017005736.2 | 35.6% | 31.5% | (many diffs) |
| 4 | human | 142686 | ASB14 | ankyrin repeat and SOCS box... | XM_017005737.2 | 35.6% | 31.5% | (many diffs) |
| 5 | human | 142686 | ASB14 | ankyrin repeat and SOCS box... | XM_024453355.1 | 35.6% | 31.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 756
- ORF length:
- 687
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgaaatgc attttgaaat tctttctcag agctctaaag atactgattc 121 cagttacgga tcttgctgcc attaagcaga gtgggatcag tccagttcac tgtgcagcag 181 caggagcaca tcctcagtgc ctggaactcc tcatccaggc tggatttgat gtgaacttca 241 tgctggatca gagaattaac aaacactacg atgaccacag gaagtcagct ttgtattttg 301 ctgtatcaaa cagtgacctc tcttcagtca agctgcttct gagtgctgga gctctgccta 361 atcaagaccc ggttaactgc ctccagatag ccctcaggat gggcaactat gagctgatca 421 gtctgctgct aaggcatggg gccaatgtca attacttctg cagagttaac cctttacatt 481 tcccatcagc actgcaatac actctgaaag atgaagtcat gctcaggatg ctgctgaact 541 atgggtatga cacagagcga tgttttgatt gcccacatgg agacaaagtc catccttcct 601 atactgttga aggctggaca tctacagtta tcaaagatac taaggtaagt ccacagtatt 661 ggctgtattc ccttacagtc attatttgtg gccctggaac tatggcaata aatagattac 721 atccactctc tccagaggct tggttgaaga gatccttgcc aactttcttg tacaaagtgg 781 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 841 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta