Construct: ORF ccsbBroad304_13222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003789.1_s304c1, BRDN0000404771
- Derived from:
- ccsbBroadEn_13222
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETFRF1 (144363)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13222
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144363 | ETFRF1 | electron transfer flavoprot... | NM_001001660.3 | 97.7% | 97.7% | 1_6delATGAAA |
| 2 | human | 144363 | ETFRF1 | electron transfer flavoprot... | XM_005253319.4 | 97.7% | 97.7% | 1_6delATGAAA |
| 3 | human | 144363 | ETFRF1 | electron transfer flavoprot... | XM_005253320.4 | 97.7% | 97.7% | 1_6delATGAAA |
| 4 | human | 144363 | ETFRF1 | electron transfer flavoprot... | XM_017018850.2 | 97.7% | 97.7% | 1_6delATGAAA |
| 5 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | NM_001163628.1 | 87.2% | 88.6% | (many diffs) |
| 6 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | NM_133688.3 | 87.2% | 88.6% | (many diffs) |
| 7 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_006506982.2 | 87.2% | 88.6% | (many diffs) |
| 8 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_006506983.3 | 87.2% | 88.6% | (many diffs) |
| 9 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_006506984.2 | 87.2% | 88.6% | (many diffs) |
| 10 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_017321721.1 | 87.2% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc caattcttta agaggagaag tactaaaact ttataaaaat ctgctgtatc 121 ttggacgaga ctatccaaaa ggagcagact attttaaaaa gcgtttgaag aacattttcc 181 ttaaaaacaa agatgtgaag aatccagaga agatcaaaga acttattgca cagggcgaat 241 ttgtaatgaa agagctagaa gctttgtact tccttaggaa atacagagct atgaaacaac 301 gctattattc agataccaac aaaactaatt gcccaacttt cttgtacaaa gtggttggta 361 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 421 ggtggcgcgt taagtcgaca atcaacctct ggatta