Construct: ORF ccsbBroad304_13263

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000050.1_s304c1, BRDN0000409343
Derived from:
ccsbBroadEn_13263
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MEI1 (150365)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13263
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 150365 MEI1 meiotic double-stranded bre... XM_011529954.2 28.6% 28.6% 1_1599del;1708_1752del;2202_2333del
2 human 150365 MEI1 meiotic double-stranded bre... XM_011529952.2 25.6% 25.6% 1_1890del;1999_2043del;2493_2624del
3 human 150365 MEI1 meiotic double-stranded bre... XM_011529949.2 23% 23% 1_2202del;2311_2355del;2805_2936del
4 human 150365 MEI1 meiotic double-stranded bre... XM_011529948.3 21.9% 21.9% 1_2367del;2476_2520del;2970_3101del
5 human 150365 MEI1 meiotic double-stranded bre... XM_011529947.2 21.3% 21.3% 1_2454del;2563_2607del;3057_3188del
6 human 150365 MEI1 meiotic double-stranded bre... XM_011529946.2 20.7% 20.7% 1_2544del;2653_2697del;3147_3278del
7 human 150365 MEI1 meiotic double-stranded bre... XM_011529943.2 19.8% 19.8% 1_2700del;2809_2853del;3303_3434del
8 human 150365 MEI1 meiotic double-stranded bre... XM_011529942.3 19.4% 19.4% 1_2784del;2893_2937del;3387_3518del
9 human 150365 MEI1 meiotic double-stranded bre... XM_011529941.2 19.3% 19.3% 1_2976del
10 human 150365 MEI1 meiotic double-stranded bre... XM_011529940.2 19.2% 19.2% 1_2871del;3429_3560del
11 human 150365 MEI1 meiotic double-stranded bre... XM_011529939.2 19.1% 19.1% 1_2976del;3085_3129del
12 human 150365 MEI1 meiotic double-stranded bre... XM_011529938.2 19% 19% 1_2859del;2968_3012del;3462_3593del
13 human 150365 MEI1 meiotic double-stranded bre... XM_011529937.2 18.9% 18.9% 1_2871del;2980_3024del;3474_3605del
14 human 150365 MEI1 meiotic double-stranded bre... NM_152513.4 18.6% 18.6% 1_2976del;3534_3665del
15 human 150365 MEI1 meiotic double-stranded bre... XM_011529935.2 18.6% 18.6% 1_2940del;3049_3093del;3543_3674del
16 human 150365 MEI1 meiotic double-stranded bre... XM_011529945.3 18.4% 18.4% 1_2976del;3085_3129del;3579_3710del
17 human 150365 MEI1 meiotic double-stranded bre... XR_937822.3 17.6% 1_3024del;3582_3713del;3871_4035del
18 human 150365 MEI1 meiotic double-stranded bre... XM_017028633.2 17.1% 17.1% 1_2976del;3167_3168ins60;3474_3605del
19 human 150365 MEI1 meiotic double-stranded bre... XM_011529936.2 16.9% 16.9% (many diffs)
20 human 150365 MEI1 meiotic double-stranded bre... XR_937817.2 13.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
780
ORF length:
714
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ggccctcacc ttggcaaagg cagattctcc caggactgca ctcctctgct
121ctgcctggct gctcactgcc tccttctctg cccagcagca caagggcagt ttgcaggttc
181accagacact ctctgtggaa atggaccaag tattgaaggc tctcagcttt ccaaagaaaa
241aggctgcact actctcagct gccatcttat gcttcctgcg gacagccctg cgacaaagct
301tttcctctgc cctggtagcc ctggtgccct caggggccca gccactgcca gccaccaagg
361acactgtcct agctccactg cgaatgtcgc aagtccggtc cctggtcatt gggctgcaga
421acctcctggt gcagaaggac cctctattgt cccaggcctg tgttggctgc ctggaggcct
481tgcttgacta cctggatgcc cggagcccag acattgctct ccacgtggcc tcccagcctt
541ggaatcggtt tttgctgttt accctcttgg atgctggaga gaattccttc ctcagacctg
601agattttgag gctcatgacc ctgctccaga gcatgggaca cctggctgac cacagcatgg
661cccagaccct gcaggcctcc ttggagggcc ttccccctag cacctcctca ggccagccac
721ccctgcagga catgctctgc ctgggagggg tggctgtatc cctgtcccac atcagaaact
781gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
841attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
901ggatta

Download FASTA (ORF) (Full)