Transcript: Human XR_937822.3

PREDICTED: Homo sapiens meiotic double-stranded break formation protein 1 (MEI1), transcript variant X30, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MEI1 (150365)
Length:
4035
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_937822.3
NBCI Gene record:
MEI1 (150365)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_937822.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000246331 CAGACCTGCAGCTAGTCTATA pLKO_005 2357 3UTR 100% 13.200 18.480 N MEI1 n/a
2 TRCN0000257508 TATCCAAGTGGGCGGTCTTAT pLKO_005 2673 3UTR 100% 13.200 18.480 N MEI1 n/a
3 TRCN0000257501 CAGCTCACAAGGTACTGATTA pLKO_005 2621 3UTR 100% 13.200 10.560 N MEI1 n/a
4 TRCN0000246332 CAGCACTCTGGAGGGATTTAG pLKO_005 1540 3UTR 100% 13.200 9.240 N MEI1 n/a
5 TRCN0000257522 TCCAATTTCCTCTACTATATG pLKO_005 1913 3UTR 100% 13.200 9.240 N MEI1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_937822.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13263 pDONR223 100% 17.6% None 1_3024del;3582_3713del;3871_4035del n/a
2 ccsbBroad304_13263 pLX_304 0% 17.6% V5 1_3024del;3582_3713del;3871_4035del n/a
3 TRCN0000465600 GACCTATTTATTGCTCGGAAATTT pLX_317 34% 17.6% V5 1_3024del;3582_3713del;3871_4035del n/a
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