Construct: ORF ccsbBroad304_13271

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007647.1_s304c1, BRDN0000407678
Derived from:
ccsbBroadEn_13271
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLB1 (151056)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13271
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151056 PLB1 phospholipase B1 XM_011532609.2 47.4% 47.4% 1_1401del;1852G>T
2 human 151056 PLB1 phospholipase B1 XM_011532610.2 47.4% 47.4% 1_1401del;1852G>T
3 human 151056 PLB1 phospholipase B1 XM_011532602.2 36.8% 36.8% 1_2169del;2620G>T
4 human 151056 PLB1 phospholipase B1 XM_011532603.2 36.8% 36.8% 1_2169del;2620G>T
5 human 151056 PLB1 phospholipase B1 XM_011532601.3 36.3% 36.2% 1_2220del;2671G>T
6 human 151056 PLB1 phospholipase B1 XM_011532593.2 29.8% 29.7% 1_2985del;3436G>T
7 human 151056 PLB1 phospholipase B1 NM_001170585.1 29.2% 29.1% 1_3072del;3523G>T
8 human 151056 PLB1 phospholipase B1 NR_138141.1 29.1% 1_2011del;2462G>T;3281_4357del
9 human 151056 PLB1 phospholipase B1 NM_153021.5 28.9% 28.9% 1_3105del;3556G>T
10 human 151056 PLB1 phospholipase B1 XM_017003433.2 28.9% 28.9% 1_3105del;3556G>T
11 human 151056 PLB1 phospholipase B1 XM_011532581.3 28.8% 28.7% 1_3132del;3583G>T
12 human 151056 PLB1 phospholipase B1 XM_017003432.2 28.7% 28.7% 1_3138del;3589G>T
13 human 151056 PLB1 phospholipase B1 XM_011532589.2 28.5% 28.5% 1_3165del;3616G>T
14 human 151056 PLB1 phospholipase B1 XM_011532579.2 28% 21.8% (many diffs)
15 human 151056 PLB1 phospholipase B1 XM_011532584.3 24.3% 23% (many diffs)
16 human 151056 PLB1 phospholipase B1 XM_011532588.2 23.1% 20.9% (many diffs)
17 human 151056 PLB1 phospholipase B1 XM_011532591.3 23% 22.5% (many diffs)
18 human 151056 PLB1 phospholipase B1 XM_011532590.2 22.9% 21% (many diffs)
19 human 151056 PLB1 phospholipase B1 XR_001738646.2 22.3% (many diffs)
20 human 151056 PLB1 phospholipase B1 XR_939663.3 20.8% (many diffs)
21 human 151056 PLB1 phospholipase B1 XM_017003434.1 18.7% 18.3% (many diffs)
22 human 151056 PLB1 phospholipase B1 XR_001738647.2 13.8% 1_3192del;3643G>T;3813_3814ins648
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1335
ORF length:
1269
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc catcacctgt cccactcaga atgagccctt cctgagaacc cctcggaata
121gtaactacac gtaccccatc aagccagcca ttgagaactg gggcagtgac ttcctgtgta
181cagagtggaa ggcttccaat agtgttccaa cctctgtcca ccagctccga ccagcagaca
241tcaaagtggt ggccgccctg ggtgactctc tgactacagc agtgggagct cgaccaaaca
301actccagtga cctacccaca tcttggaggg gactctcttg gagcattgga ggggatggga
361acttggagac tcacaccaca ctgcccaaca ttctgaagaa gttcaaccct tacctccttg
421gcttctctac cagcacctgg gaggggacag caggactaaa tgtggcagcg gaaggggcca
481gagctaggga catgccagcc caggcctggg acctgttaga gcgaatgaaa aacagccccg
541acatcaacct ggagaaagac tggaagctgg tcacactctt cattggggtc aacgacttgt
601gtcattactg tgagaatccg gaggcccact tggccacgga atatgttcag cacatccaac
661aggccctgga catcctctct gaggagctcc caagggcttt cgtcaacgtg gtggaggtca
721tggagctggc tagcctgtac cagggccaag gcgggaaatg tgccatgctg gcagctcaga
781acaactgcac ttgcctcaga cactcgcaaa gctccctgga gaagcaagaa ctgaagaaag
841tgaactggaa cctccagcat ggcatctcca gtttctccta ctggcaccaa tacacacagc
901gtgaggactt tgcggttgtg gtgcagcctt tcttccaaaa cacactcacc ccactgaacg
961agagagggga cactgacctc accttcttct ccgaggactg ttttcacttc tcagaccgcg
1021ggcatgccga gatggccatc gcactctgga acaacatgct ggaaccagtg ggccgcaaga
1081ctacctccaa caacttcacc cacagccgag ccaaactcaa gtgcccctct cctgagagcc
1141cttacctcta caccctgcgg aacagccgat tgctcccaga ccaggctgaa gaagcccccg
1201aggtgctcta ctgggctgtc ccagtggcag cgggagtcgg ccttgtggtg ggcatcatcg
1261ggacagtggt ctggaggtgc aggagaggtg gccggaggga agatcctcca atgagcctgc
1321gcactgtggc cctctaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1381ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1441cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)