Transcript: Human XR_001738646.2

PREDICTED: Homo sapiens phospholipase B1 (PLB1), transcript variant X13, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PLB1 (151056)
Length:
5422
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001738646.2
NBCI Gene record:
PLB1 (151056)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001738646.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156689 GACCTGGTAGAGCGAATGAAA pLKO.1 3868 3UTR 100% 5.625 7.875 N PLB1 n/a
2 TRCN0000158235 CAGAGCCCTTTGGACCAATAT pLKO.1 3398 3UTR 100% 13.200 9.240 N PLB1 n/a
3 TRCN0000155628 CCTAATATCCTTCGGGAGTTT pLKO.1 2724 3UTR 100% 4.950 3.465 N PLB1 n/a
4 TRCN0000156446 CCATGAAGACTGGAAGGTCAT pLKO.1 2900 3UTR 100% 4.050 2.835 N PLB1 n/a
5 TRCN0000156549 GACTTGTGGATTCAGGCTCAA pLKO.1 720 3UTR 100% 4.050 2.835 N PLB1 n/a
6 TRCN0000154756 GCATTCCTCAATCAAGCTGTT pLKO.1 2799 3UTR 100% 4.050 2.835 N PLB1 n/a
7 TRCN0000150904 GAATATGTTCAGCACATCCAA pLKO.1 3997 3UTR 100% 3.000 2.100 N PLB1 n/a
8 TRCN0000157010 GCTGAGGATCTTATGAGCCAA pLKO.1 2832 3UTR 100% 2.640 1.848 N PLB1 n/a
9 TRCN0000154417 GCTCCTGGAATGGATACATTT pLKO.1 4858 3UTR 100% 13.200 7.920 N PLB1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001738646.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13272 pDONR223 100% 26.5% None (many diffs) n/a
2 ccsbBroad304_13272 pLX_304 0% 26.5% V5 (many diffs) n/a
3 TRCN0000478882 TAATACGAAGGTCCCAGTACGGTC pLX_317 20.3% 26.5% V5 (many diffs) n/a
4 ccsbBroadEn_13271 pDONR223 100% 22.3% None (many diffs) n/a
5 ccsbBroad304_13271 pLX_304 0% 22.3% V5 (many diffs) n/a
6 TRCN0000491336 TTTCTCGGACTGAAACCGATAAGC pLX_317 20.1% 22.3% V5 (many diffs) n/a
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