Construct: ORF ccsbBroad304_13272

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012561.1_s304c1, BRDN0000403932
Derived from:
ccsbBroadEn_13272
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLB1 (151056)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13272
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151056 PLB1 phospholipase B1 XM_011532608.3 59.5% 59.5% 1_996del
2 human 151056 PLB1 phospholipase B1 XM_011532607.2 52.8% 51.3% (many diffs)
3 human 151056 PLB1 phospholipase B1 XM_011532606.3 52% 50.5% (many diffs)
4 human 151056 PLB1 phospholipase B1 XM_011532602.2 41.8% 40.7% (many diffs)
5 human 151056 PLB1 phospholipase B1 XM_011532603.2 41.8% 40.7% (many diffs)
6 human 151056 PLB1 phospholipase B1 XM_011532601.3 41.2% 40.1% (many diffs)
7 human 151056 PLB1 phospholipase B1 XR_001738647.2 37.7% (many diffs)
8 human 151056 PLB1 phospholipase B1 XM_017003434.1 35.7% 34.7% (many diffs)
9 human 151056 PLB1 phospholipase B1 XM_011532591.3 34.3% 33.4% (many diffs)
10 human 151056 PLB1 phospholipase B1 XM_011532590.2 34.2% 33.2% (many diffs)
11 human 151056 PLB1 phospholipase B1 XM_011532588.2 34.1% 33.2% (many diffs)
12 human 151056 PLB1 phospholipase B1 XM_011532593.2 33.8% 32.9% (many diffs)
13 human 151056 PLB1 phospholipase B1 XM_011532584.3 33.1% 32.2% (many diffs)
14 human 151056 PLB1 phospholipase B1 NM_153021.5 32.9% 32% (many diffs)
15 human 151056 PLB1 phospholipase B1 XM_017003433.2 32.9% 32% (many diffs)
16 human 151056 PLB1 phospholipase B1 XM_011532581.3 32.7% 31.8% (many diffs)
17 human 151056 PLB1 phospholipase B1 XM_017003432.2 32.6% 31.7% (many diffs)
18 human 151056 PLB1 phospholipase B1 XM_011532579.2 32.5% 31.6% (many diffs)
19 human 151056 PLB1 phospholipase B1 XM_011532589.2 32.4% 31.5% (many diffs)
20 human 151056 PLB1 phospholipase B1 XR_939663.3 32% (many diffs)
21 human 151056 PLB1 phospholipase B1 NM_001170585.1 31.1% 30.2% (many diffs)
22 human 151056 PLB1 phospholipase B1 NR_138141.1 27.2% (many diffs)
23 human 151056 PLB1 phospholipase B1 XR_001738646.2 26.5% (many diffs)
24 human 151056 PLB1 phospholipase B1 XM_011532609.2 19.5% 25.8% (many diffs)
25 human 151056 PLB1 phospholipase B1 XM_011532610.2 19.5% 25.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1530
ORF length:
1464
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gccagcagga gagaaagatg agccattgag tgtaaaacac gggaggccaa
121tgaagtgtcc ctctcaggag agcccctatc tgttcagcta cagaaacagc aactacctga
181ccagactgca gaaaccccaa gacaagcttg aggtaagaga aggagcggaa atcagatgtc
241ctgacaaaga cccctccgat acggttccca cctcagttca taggctgaag ccggctgaca
301tcaacgtaat tggagccctg ggtgactctc tcacggcagg caatggggcc gggtccacac
361ctgggaacgt cttggacgtc ttgactcagt accgaggcct gtcctggagc gtcggcggag
421atgagaacat cggcaccgtt accaccctgg cgaacatcct ccgggaattc aacccttccc
481tgaagggctt ctctgttggc actgggaaag aaaccagtcc taatgccttc ttaaaccagg
541ctgtggcagg aggccgagct gaggatctac ctgtccaggc caggaggctg gtggacctga
601tgaagaatga cacgaggata cactttcagg aagactggaa gataataacc ctgtttatag
661gcggcaatga cctctgtgat ttctgcaatg atctggtcca ctattctccc cagaacttca
721cagacaacat tggaaaggcc ctggacatcc tccatgctga ggttcctcgg gcatttgtga
781acctggtgac ggtgcttgag atcgtcaacc tgagggagct gtaccaggag aaaaaagtct
841actgcccaag gatgatcctc aggtctctgt gtccctgtgt cctgaagttt gatgataact
901caacagaact tgctaccctc atcgaattca acaagaagtt tcaggagaag acccaccaac
961tgattgagag tgggcgatat gacacaaggg aagattttac tgtggttgtg cagccgttct
1021ttgaaaacgt ggacatgcca aagacctcgg aaggattgcc tgacaactct ttcttcgctc
1081ctgactgttt ccacttcagc agcaagtctc actcccgagc agccagtgct ctctggaaca
1141atatgctgga gcctgttggc cagaagacga ctcgtcataa gtttgaaaac aagatcaata
1201tcacatgtcc gaaccaggtc cagccgtttc tgaggaccta caagaacagc atgcagggtc
1261atgggacctg gctgccatgc agggacagag ccccttctgc cttgcaccct acctcagtgc
1321atgccctgag acctgcagac atccaagttg tggctgctct gggggattct ctgaccgctg
1381gcaatggaat tggctccaaa ccagacgacc tccccgatgt caccacacag tatcggggac
1441tgtcatacag agaaagtaaa ccagggttct tatcagactc ctgggtcagc aaatccaaca
1501ggaaatgcac cagaaaagca ccaaatccct gcccaacttt cttgtacaaa gtggttggta
1561agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1621ggtggcgcgt taagtcgaca atcaacctct ggatta

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