Construct: ORF ccsbBroad304_13299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016123.1_s304c1, BRDN0000410850
- Derived from:
- ccsbBroadEn_13299
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS37D (155382)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13299
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155382 | VPS37D | VPS37D subunit of ESCRT-I | XM_017011779.1 | 69% | 69% | 1_195del |
| 2 | human | 155382 | VPS37D | VPS37D subunit of ESCRT-I | NM_001077621.2 | 57.7% | 57.7% | 1_318del |
| 3 | mouse | 194309 | Vps37d | vacuolar protein sorting 37D | NM_177574.4 | 70% | 68.7% | (many diffs) |
| 4 | mouse | 194309 | Vps37d | vacuolar protein sorting 37D | XM_011240873.2 | 55.4% | 58.9% | (many diffs) |
| 5 | mouse | 194309 | Vps37d | vacuolar protein sorting 37D | XM_011240872.2 | 51% | 54.3% | (many diffs) |
| 6 | mouse | 194309 | Vps37d | vacuolar protein sorting 37D | NM_001199677.1 | 49.6% | 52.8% | (many diffs) |
| 7 | mouse | 194309 | Vps37d | vacuolar protein sorting 37D | XR_001784645.1 | 25.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tcgctggagt ccccactgcg cgctgggctg gctgcaggct gagctagaag 121 aggcggagca ggaggcagag gagcagatgg agcagctgct gctcggggag caaagcctgg 181 aggccttcct gcctgccttc cagcgtggcc gcgccctggc ccacctgagg cggacgcagg 241 cagagaagct gcaggagctg ctgcggcgtc gggagcgttc tgcccagccg gcccccacct 301 cggctgctga tccccccaaa tccttcccgg ctgcagctgt cctgcccact ggggccgccc 361 gggggccacc agcagtgccc cggagcctgc cccccttgga ctcccgccca gtgcccccac 421 tgaagggctc ccccgggtgc cccctcggcc cggcccccct gctgagccct cggccctcgc 481 agccagagcc cccccaccgg tacccaactt tcttgtacaa agtggttggt aagcctatcc 541 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 601 ttaagtcgac aatcaacctc tggatta