Construct: ORF ccsbBroad304_13344
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013224.1_s304c1, BRDN0000407934
- Derived from:
- ccsbBroadEn_13344
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRA3 (166647)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13344
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 166647 | ADGRA3 | adhesion G protein-coupled ... | XM_005248137.5 | 45% | 44% | (many diffs) |
| 2 | human | 166647 | ADGRA3 | adhesion G protein-coupled ... | XM_011513811.2 | 34.5% | 34.3% | (many diffs) |
| 3 | human | 166647 | ADGRA3 | adhesion G protein-coupled ... | NM_145290.4 | 28.4% | 27.7% | (many diffs) |
| 4 | human | 166647 | ADGRA3 | adhesion G protein-coupled ... | XR_001741153.1 | 23.9% | (many diffs) | |
| 5 | mouse | 70693 | Adgra3 | adhesion G protein-coupled ... | NM_133911.1 | 24.6% | 26.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc actgaagagg aataactcta aataccctcc gcttgaattg ccgtctttct 121 acatgactcc atctcatcgc caagttgtgt ttgaaggaga cagccttcct ttccagtgca 181 tggcttcata tattgatcag gacatgcaag tgttgtggta tcaggatggg agaatagttg 241 aaaccgatga atcgcaaggt atttttgttg aaaagaacat gattcacaac tgctccttga 301 ttgcaagtgc cctaaccatt tctaatattc aggctggatc tactggaaat tggggctgtc 361 atgtccagac caaacgtggg aataatacga ggactgtgga tattgtggta ttagagagtt 421 ctgcacagta ctgtccgcca gagagggtgg taaacaacaa aggtgacttc agatggccca 481 gaacattggc aggcattact gcatatctgc agtgtacgcg gaacacccat ggcagtggga 541 tatatcccgg aaacccacag gatgagagaa aagcttggcg cagatgtgat agaggtggct 601 tttgggcaga tgatgattat tctcgctgtc agtatgcaaa tgatgtcact agagttcttt 661 atatgtttaa tcagatgccc ctcaatctta ccaatgccgt ggcaacagct cgacagttac 721 tggcttacac tgtggaagca gccaactttt ctgacaaaat ggatgttata tttgtggcag 781 aaatgattga aaaatttgga agatttacca aggaggaaaa atcaaaagag ctaggtgacg 841 tgatggttga cattgcaagt aacatcatgt tggctgatga acgtgtcctg tggctggcgc 901 agagggaagc taaagcctgc agtaggattg tgcagtgtct tcagcgcatt gctacctacc 961 ggctagccgg tggagctcac gtttattcaa catatccacc caatattgct ctggaagctt 1021 atgtcatcaa gtctactggc ttcacgggga tgacctgtac cgtgttccag aaagtggcag 1081 cctctgatcg tacaggactt tcggattatg ggaggcggga tccagaggga aacctggata 1141 agcagctgag ctttaagtgc aatgtttcaa atacattttc gagtctggca ctaaagggga 1201 aattccggtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1261 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1321 tcaacctctg gatta