Construct: ORF ccsbBroad304_13418

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002026.1_s304c1, BRDN0000403149
Derived from:
ccsbBroadEn_13418
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RASGEF1A (221002)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13418
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221002 RASGEF1A RasGEF domain family member 1A XM_005271809.3 93.5% 93.4% 0_1ins81;501C>A;1153A>G
2 human 221002 RASGEF1A RasGEF domain family member 1A XM_011539500.2 93.5% 93.4% 0_1ins81;501C>A;1153A>G
3 human 221002 RASGEF1A RasGEF domain family member 1A NM_145313.4 88.8% 88.7% 1_159del;741C>A;1393A>G
4 human 221002 RASGEF1A RasGEF domain family member 1A NM_001282862.1 87.3% 87.3% 1_183del;765C>A;1417A>G
5 mouse 70727 Rasgef1a RasGEF domain family, membe... NM_001316749.1 79.4% 84.4% (many diffs)
6 mouse 70727 Rasgef1a RasGEF domain family, membe... XM_017321738.1 79.4% 84.4% (many diffs)
7 mouse 70727 Rasgef1a RasGEF domain family, membe... NM_027526.1 78.1% 83% (many diffs)
8 mouse 70727 Rasgef1a RasGEF domain family, membe... XM_017321737.1 77.7% 82.5% (many diffs)
9 mouse 70727 Rasgef1a RasGEF domain family, membe... XM_006506596.3 68.1% 67.9% (many diffs)
10 mouse 70727 Rasgef1a RasGEF domain family, membe... XM_006506597.3 68.1% 67.9% (many diffs)
11 mouse 70727 Rasgef1a RasGEF domain family, membe... XM_006506595.3 67.1% 66.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1350
ORF length:
1284
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gcaccttgtt cccacggtgg actattaccc cgataggacg tacatcttca
121cctttctcct gagctcccgg gtctttatgc cccctcatga cctgctggcc cgcgtggggc
181agatctgcgt ggagcagaag cagcagctgg aagccgggcc tgaaaaggcc aagctgaagt
241ctttctcagc caagatcgtg cagctcctga aggagtggac cgaggccttc ccctatgact
301tccaggatga gaaggccatg gccgagctga aagccatcac acaccgtgtc acccagtgtg
361atgaggagaa tggcacagtg aagaaggcca ttgcccagat gacacagagc ctgttgctgt
421ccttggctgc ccggagccag ctccaggaac tgcgagagaa gctccggcca ccggctgtag
481acaaggggcc catcctcaag accaagccac cagccgccca gaaggacatc ctgggcgtgt
541gctgcgaccc cctggtgctg gcccagcagc tgactcacat tgagctggac agggtcagca
601gcatttaccc tgaggacttg atgcagatcg tcagccacat ggactcattg gacaaccaca
661ggtgccgagg ggacctgacc aagacctaca gcctggaggc ctatgacaac tggttcaact
721gcctgagcat gctggtggcc actgaggtgt gccgggtggt gaagaagaaa caccggaccc
781gcatgttgga gttcttcatt gatgtggccc gggagtgctt caacatcggg aacttcaact
841ccatgatggc catcatctct ggcatgaacc tcagtcctgt ggcaaggctg aagaaaactt
901ggtccaaggt caagacagcc aagtttgatg tcttggagca tcacatggac ccgtccagca
961acttctgcaa ctaccgtaca gccctgcagg gggccacgca gaggtcccag atggccaaca
1021gcagccgtga aaagatcgtc atccctgtgt tcaacctctt cgttaaggac atctacttcc
1081tgcacaaaat ccataccaac cacctgccca acgggcacat taactttaag aaattctggg
1141agatctccag acagatccat gagttcatga catggacaca ggtagagtgt cctttcgaga
1201aggacaagaa gattcagagt tacctgctca cggcgcccat ctacagcgag gaagctctct
1261tcgtcgcctc ctttgaaagt gagggtcccg agaaccacgt ggaaaaagac agctggaaga
1321ccctcaggac cacccttctg aacagagcct gcccaacttt cttgtacaaa gtggttggta
1381agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1441ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)