Construct: ORF ccsbBroad304_13434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004632.1_s304c1, BRDN0000409730
- Derived from:
- ccsbBroadEn_13434
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM185A (222234)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13434
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222234 | FAM185A | family with sequence simila... | NM_001350987.1 | 38% | 38% | 432_1134delinsT |
| 2 | human | 222234 | FAM185A | family with sequence simila... | NM_001145268.2 | 36.5% | 36.4% | 179G>C;432_1176delinsT |
| 3 | human | 222234 | FAM185A | family with sequence simila... | NR_146977.1 | 21.5% | 1_256del;689_2004del | |
| 4 | human | 222234 | FAM185A | family with sequence simila... | NR_146987.1 | 21.2% | 1_256del;688_2026delinsT | |
| 5 | human | 222234 | FAM185A | family with sequence simila... | NR_146985.1 | 19.6% | 1_256del;689_2194del | |
| 6 | human | 222234 | FAM185A | family with sequence simila... | NR_146989.1 | 18.9% | 1_256del;689_2274del | |
| 7 | human | 222234 | FAM185A | family with sequence simila... | NR_146980.1 | 18.6% | (many diffs) | |
| 8 | human | 222234 | FAM185A | family with sequence simila... | NR_146986.2 | 15.7% | 1_209del;388G>C;642_2735del | |
| 9 | human | 641808 | FAM185BP | family with sequence simila... | NR_146190.1 | 12.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tgccccctgc tcaggttggg agcttggctg cttccgtctc tgtctccgtc 121 aggtccgact gtgggctggc gctgggcgct gggcttgctg ggcttgccaa gccaggccgt 181 acagctcagg tgggagcgag cgctggcccg gatcggagac tgaggtccct ccgcctggcc 241 cggcgcgccg aactctgaag gagtggacac tgcaggtgag cccgtttggt cggctgcggg 301 cgcggctccc gtgccacctg gccgtgaggc ccctggaccc cctcacctac ccggatggcg 361 accgcgtgct ggtcgcggtg tgcggcgtgg agggcggcgt gcggggcctg gacggcctgc 421 aggtgaagta cgacgaggat ctggaggaga tggccattgt gtctgatact atccaccccc 481 aggcgtccgt ggaggtttac ccaactttct tgtacaaagt ggttggtaag cctatcccta 541 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 601 agtcgacaat caacctctgg atta