Construct: ORF ccsbBroad304_13487

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008591.1_s304c1, BRDN0000411625
Derived from:
ccsbBroadEn_13487
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMIE (259236)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13487
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 259236 TMIE transmembrane inner ear NM_147196.3 99.3% 99.3% 367_369delAAG
2 human 259236 TMIE transmembrane inner ear NM_001370524.1 65.3% 65.3% 0_1ins159;208_210delAAG
3 human 259236 TMIE transmembrane inner ear NM_001370525.1 65.3% 65.3% 0_1ins159;208_210delAAG
4 mouse 20776 Tmie transmembrane inner ear NM_146260.2 87.8% 91.6% (many diffs)
5 mouse 20776 Tmie transmembrane inner ear XM_006512007.3 87.8% 91.6% (many diffs)
6 mouse 20776 Tmie transmembrane inner ear XM_006512008.3 87.8% 91.6% (many diffs)
7 mouse 20776 Tmie transmembrane inner ear XM_006512009.3 87.8% 91.6% (many diffs)
8 mouse 20776 Tmie transmembrane inner ear XM_006512010.3 87.8% 91.6% (many diffs)
9 mouse 20776 Tmie transmembrane inner ear XM_006512013.3 87.8% 91.6% (many diffs)
10 mouse 20776 Tmie transmembrane inner ear XM_011242944.2 87.8% 91.6% (many diffs)
11 mouse 20776 Tmie transmembrane inner ear XM_011242945.2 87.8% 91.6% (many diffs)
12 mouse 20776 Tmie transmembrane inner ear XM_006512014.3 59.7% 63.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
534
ORF length:
465
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggggtgg ccgggcgcgg gtcccctctg cgtgctgggc ggcgccgcac
121tcggggtgtg cctcgcgggg gttgccgggc agctggtgga gcccagcacg gccccaccca
181agcccaagcc gcctccgctg accaaggaga cagtggtgtt ctgggacatg cgcctgtggc
241acgtggtggg catcttttcg ctcttcgtgt tgtccatcat catcacgctg tgctgtgtct
301tcaactgtcg tgtgccacgg acccggaagg agatcgaagc ccggtacctg cagcgaaagg
361cagccaagat gtacacagac aagctggaga ctgtgccacc cctcaatgag ctcacagaag
421tcccaggaga ggataagaag aagaagaaga agaagaagga cagtgtggac acagtggcca
481tcaaagtaga ggaggatgag aagaatgagg ccaagaagaa gaaaggagag aaattgccaa
541ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
601cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)