Construct: ORF ccsbBroad304_13599

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007230.1_s304c1, BRDN0000460551
Derived from:
ccsbBroadEn_13599
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACER2 (340485)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13599
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 340485 ACER2 alkaline ceramidase 2 XM_005251448.3 100% 100%
2 human 340485 ACER2 alkaline ceramidase 2 XM_017014694.1 100% 100%
3 human 340485 ACER2 alkaline ceramidase 2 NM_001010887.3 82.1% 82.1% 1_147del
4 human 340485 ACER2 alkaline ceramidase 2 XM_011517859.2 81.8% 81.8% 0_1ins123
5 human 340485 ACER2 alkaline ceramidase 2 XR_242506.2 53.2% 1_305del;799_907del;995_996ins97
6 human 340485 ACER2 alkaline ceramidase 2 XR_002956781.1 47.8% 1_305del;799_1040del;1133_1134ins92
7 human 340485 ACER2 alkaline ceramidase 2 XR_002956780.1 47.7% 1_305del;799_1043del;1136_1137ins92
8 human 340485 ACER2 alkaline ceramidase 2 XM_011517858.1 47.5% 42% (many diffs)
9 human 340485 ACER2 alkaline ceramidase 2 XR_242507.3 11.9% 1_305del;799_934del;1120_5659del
10 mouse 230379 Acer2 alkaline ceramidase 2 NM_139306.3 72% 76% (many diffs)
11 mouse 230379 Acer2 alkaline ceramidase 2 XM_006537877.3 61.3% 50.8% (many diffs)
12 mouse 230379 Acer2 alkaline ceramidase 2 NM_001290541.1 58.3% 61.8% (many diffs)
13 mouse 230379 Acer2 alkaline ceramidase 2 NM_001290543.1 53.5% 56.3% (many diffs)
14 mouse 230379 Acer2 alkaline ceramidase 2 XM_006537878.3 48.8% 38% (many diffs)
15 mouse 230379 Acer2 alkaline ceramidase 2 XM_011250012.2 47.3% 50.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
747
ORF length:
678
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtgcttgttt cgtcagtatg caacatgctt caacagtggc atctacttaa
121tctggactct tttggttgta gtgggaattg gatccgtcta cttccatgca acccttagtt
181tcttgggtca gatgcttgat gaacttgcag tcctttgggt tctgatgtgt gctttggcca
241tgtggttccc cagaaggtat ctaccaaaga tctttcggaa tgaccggggt aggttcaagg
301tggtggtcag tgtcctgtct gcggttacga cgtgcctggc atttgtcaag cctgccatca
361acaacatctc tctgatgacc ctgggagttc cttgcactgc actgctcatc gcagagctaa
421agaggtgtga caacatgcgt gtgtttaagc tgggcctctt ctcgggcctc tggtggaccc
481tggccctgtt ctgctggatc agtgaccgag ctttctgcga gctgctgtca tccttcaact
541tcccctacct gcactgcatg tggcacatcc tcatctgcct tgctgcctac ctgggctgtg
601tatgctttgc ctactttgat gctgcctcag agattcctga gcaaggccct gtcatcaagt
661tctggcccaa tgagaaatgg gccttcattg gtgtccccta tgtgtccctc ctgtgtgcca
721acaagaaatc atcagtcaag atcacgttgc caactttctt gtacaaagtg gttggtaagc
781ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
841ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)