Construct: ORF ccsbBroad304_13612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007215.1_s304c1, BRDN0000461944
- Derived from:
- ccsbBroadEn_13612
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF8L2 (347442)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13612
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | NM_001353448.2 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 2 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | NM_001353449.2 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 3 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | NM_001353450.2 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 4 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452371.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 5 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452372.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 6 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452373.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 7 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452374.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 8 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452375.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 9 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452376.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 10 | human | 347442 | DCAF8L2 | DDB1 and CUL4 associated fa... | XM_024452377.1 | 99.5% | 99.5% | 388_396delGAGGAGGAG |
| 11 | human | 139425 | DCAF8L1 | DDB1 and CUL4 associated fa... | NM_001017930.2 | 85.1% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1953
- ORF length:
- 1884
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccaccaa gaaggcagca cagacggctt accagactta gggactgaaa 121 gcctgttcag cagcccagag gagcagtctg gagccgtggc ggcgacagag gcctcctcag 181 acattgacat agcgacctca gagctgagtg tgacagtgac cggagatggc agtgatagca 241 gggatggtgg attccccaac gatgccagca cagaaaatcg aagctcagac caagaaagcg 301 caagtgaaga catcgaactt gagagcttgg aggactttga gcatttcctc atgagtggtg 361 aaagtttatt ccattaccct ttagtgggag aggaggagac agaaagggag gaggaagacg 421 aagagataca agaggaggga ggggaggagg aggaagagga ggaggaggag gaggaggagg 481 aggaggagga ggaagaagaa cagcctcggg cgggtccaca aggcagtggc ggcaaccatg 541 agcagtattc gttagaggag gatcaggcgc tggaggagtg ggtttcctca gagacatctg 601 ccctgccccg acctcgctgg caggtcgtta ctgctcttca ccagcggcag ctgggttcac 661 gtccccgctt tgtatatgag gcctgtgggg caagagcctt tgtgcagcgt ttccgcctgc 721 aatatcgtct tgcagaccat gtcggctgtg tcaatactgt acactttaac cagcgtggca 781 cccggctggc cagtagcggt gatgacctaa aggtgatagt gtgggactgg gtgcggcaga 841 ggccagtact gaactttgaa agtggtcaca caaataatgt cttccaggcc aagttccttc 901 ctaactgtgg tgattccact ctggccatgt gtgcccgtga tgggcaggta cgggtagcag 961 agttaattaa tgcatcatat ttcaacaata ctaagtgtgt ggcccagcac aggggacctg 1021 cccacaagtt ggctctggag cctgactctc cttataagtt cctcacttca ggtgaagatg 1081 ctgttgtctt caccattgac ctcagacaag accggccagc ttcaaaagtt gtggtaacaa 1141 gagaaaatga taagaaagtg ggactgtata caattactgt gaatcccgcc aatacctacc 1201 aatttgcagt gggtggacaa gatcagtttg taaggattta tgaccagagg aaaattgata 1261 agaaagaaaa caatggcgtg ctcaagaaat tcactcctca tcatctggtt aattgtgatt 1321 tcccaacaaa catcacctgc gttgtgtaca gccacgatgg cacagagctg ctagccagct 1381 acaatgatga cgatatttac ctcttcaact cctctcacag tgatggtgct caatacagta 1441 agagatttaa gggacacaga aataatacca cagtcaaagg tgttaatttc tatggcccca 1501 ggagtgagtt tgtagtgagc ggtagtgatt gcgggcacat cttcttctgg gagaaatcat 1561 cctgccaaat catccagttc ctaaagggga gcagagaagg tacaataaac tgtcttgaac 1621 cccaccctta cctacctgtg ttggcgtgca gtggcctaga tcatgatgtc aagatctgga 1681 cacccacagc taaagctgcc actgagctta ctgggttaaa gaaggtgatt aagaagaaca 1741 agtgggaacg agatgaagac agcttgcacc atggcagcct gtttgaccag tacatgcttt 1801 ggttcctcct gcgtcacgtg acgcagagag gtcgtcacca ggactggaga agtggtgaag 1861 ctgaatttcc agatgaagaa tcggatgagt cttccagcac ttcagagaca tctgaggagg 1921 aggtccaaga ccgagtgcag tgcatgccat ccttgccaac tttcttgtac aaagtggttg 1981 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2041 accggtggcg cgttaagtcg acaatcaacc tctggatta