Construct: ORF ccsbBroad304_13612

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007215.1_s304c1, BRDN0000461944
Derived from:
ccsbBroadEn_13612
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCAF8L2 (347442)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13612
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... NM_001353448.2 99.5% 99.5% 388_396delGAGGAGGAG
2 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... NM_001353449.2 99.5% 99.5% 388_396delGAGGAGGAG
3 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... NM_001353450.2 99.5% 99.5% 388_396delGAGGAGGAG
4 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452371.1 99.5% 99.5% 388_396delGAGGAGGAG
5 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452372.1 99.5% 99.5% 388_396delGAGGAGGAG
6 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452373.1 99.5% 99.5% 388_396delGAGGAGGAG
7 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452374.1 99.5% 99.5% 388_396delGAGGAGGAG
8 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452375.1 99.5% 99.5% 388_396delGAGGAGGAG
9 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452376.1 99.5% 99.5% 388_396delGAGGAGGAG
10 human 347442 DCAF8L2 DDB1 and CUL4 associated fa... XM_024452377.1 99.5% 99.5% 388_396delGAGGAGGAG
11 human 139425 DCAF8L1 DDB1 and CUL4 associated fa... NM_001017930.2 85.1% 76.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1953
ORF length:
1884
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcccaccaa gaaggcagca cagacggctt accagactta gggactgaaa
121gcctgttcag cagcccagag gagcagtctg gagccgtggc ggcgacagag gcctcctcag
181acattgacat agcgacctca gagctgagtg tgacagtgac cggagatggc agtgatagca
241gggatggtgg attccccaac gatgccagca cagaaaatcg aagctcagac caagaaagcg
301caagtgaaga catcgaactt gagagcttgg aggactttga gcatttcctc atgagtggtg
361aaagtttatt ccattaccct ttagtgggag aggaggagac agaaagggag gaggaagacg
421aagagataca agaggaggga ggggaggagg aggaagagga ggaggaggag gaggaggagg
481aggaggagga ggaagaagaa cagcctcggg cgggtccaca aggcagtggc ggcaaccatg
541agcagtattc gttagaggag gatcaggcgc tggaggagtg ggtttcctca gagacatctg
601ccctgccccg acctcgctgg caggtcgtta ctgctcttca ccagcggcag ctgggttcac
661gtccccgctt tgtatatgag gcctgtgggg caagagcctt tgtgcagcgt ttccgcctgc
721aatatcgtct tgcagaccat gtcggctgtg tcaatactgt acactttaac cagcgtggca
781cccggctggc cagtagcggt gatgacctaa aggtgatagt gtgggactgg gtgcggcaga
841ggccagtact gaactttgaa agtggtcaca caaataatgt cttccaggcc aagttccttc
901ctaactgtgg tgattccact ctggccatgt gtgcccgtga tgggcaggta cgggtagcag
961agttaattaa tgcatcatat ttcaacaata ctaagtgtgt ggcccagcac aggggacctg
1021cccacaagtt ggctctggag cctgactctc cttataagtt cctcacttca ggtgaagatg
1081ctgttgtctt caccattgac ctcagacaag accggccagc ttcaaaagtt gtggtaacaa
1141gagaaaatga taagaaagtg ggactgtata caattactgt gaatcccgcc aatacctacc
1201aatttgcagt gggtggacaa gatcagtttg taaggattta tgaccagagg aaaattgata
1261agaaagaaaa caatggcgtg ctcaagaaat tcactcctca tcatctggtt aattgtgatt
1321tcccaacaaa catcacctgc gttgtgtaca gccacgatgg cacagagctg ctagccagct
1381acaatgatga cgatatttac ctcttcaact cctctcacag tgatggtgct caatacagta
1441agagatttaa gggacacaga aataatacca cagtcaaagg tgttaatttc tatggcccca
1501ggagtgagtt tgtagtgagc ggtagtgatt gcgggcacat cttcttctgg gagaaatcat
1561cctgccaaat catccagttc ctaaagggga gcagagaagg tacaataaac tgtcttgaac
1621cccaccctta cctacctgtg ttggcgtgca gtggcctaga tcatgatgtc aagatctgga
1681cacccacagc taaagctgcc actgagctta ctgggttaaa gaaggtgatt aagaagaaca
1741agtgggaacg agatgaagac agcttgcacc atggcagcct gtttgaccag tacatgcttt
1801ggttcctcct gcgtcacgtg acgcagagag gtcgtcacca ggactggaga agtggtgaag
1861ctgaatttcc agatgaagaa tcggatgagt cttccagcac ttcagagaca tctgaggagg
1921aggtccaaga ccgagtgcag tgcatgccat ccttgccaac tttcttgtac aaagtggttg
1981gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2041accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)