Construct: ORF ccsbBroad304_13632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004650.1_s304c1, BRDN0000400691
- Derived from:
- ccsbBroadEn_13632
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIA3 (375056)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13632
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | NM_001300867.1 | 58.6% | 58.4% | 1_972del;1609G>T;1801A>G |
| 2 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | NM_001324065.1 | 56.9% | 56.7% | (many diffs) |
| 3 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | NM_001324064.1 | 26.4% | 26.3% | 1_3846del;4483G>T;4675A>G |
| 4 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | XM_017001243.2 | 26% | 25.9% | (many diffs) |
| 5 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | NM_001324063.1 | 24.9% | 24.8% | 1_4161del;4798G>T;4990A>G |
| 6 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | XM_006711304.4 | 24.5% | 24.5% | (many diffs) |
| 7 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | NM_198551.4 | 24.1% | 24% | 1_4338del;4975G>T;5167A>G |
| 8 | human | 375056 | MIA3 | MIA SH3 domain ER export fa... | NM_001324062.1 | 23.8% | 23.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1449
- ORF length:
- 1383
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa aaatcaaatt aagcagatga tggatgtctc tcggacacag actgcaatat 121 cggtagttga agaggatcta aagcttttac agcttaagct aagagcctcc gtgtccacta 181 aatgtaacct ggaagaccag gtaaagaaat tggaagatga ccgcaactca ctacaagctg 241 ccaaagctgg actggaagat gaatgcaaaa ccttgaggca gaaagtggag attctgaatg 301 agctctatca gcagaaggag atggctttgc aaaagaaact gagtcaagaa gagtatgaac 361 ggcaagaaag agagcacagg ctgtcagctg cagatgaaaa ggcagtttcg gctgcagagg 421 aagtaaaaac ttacaagcgg agaattgaag aaatggagga tgaattacag aagacagagc 481 ggtcatttaa aaaccagatc gctacccatg agaagaaagc tcatgaaaac tggctcaaag 541 ctcgtgctgc agaaagagct atagctgaag agaaaaggga agctgccaat ttgagacaca 601 aattattaga attaacacaa aagatggcaa tgctgcaaga agaacctgtg attgtaaaac 661 caatgccagg aaaaccaaat acacaaaacc ctccacggag atgtcctctg agccagaatg 721 gctcttttgg cccatcccct gtgagtggtg gagaatgctc ccctccattg acagtggagc 781 cacccgtgag acctctctct gctactctca atcgaagaga tatgcctaga agtgaatttg 841 gatcagtgga cgggcctcta cctcatcctc gatggtcagc tgaggcatct ggggaaccct 901 ctccttctga tccaggatct ggtacagcta ccatgatgaa cagcagctca agaggctctt 961 cccctaccag ggtactcgat gaaggcaagg ttaatatggc tccaaaaggg ccccctcctt 1021 tcccaggagt ccctctcatg agcaccccca tgggaggccc tgtaccacca cccattcgat 1081 atggaccacc acctcagctc tgcggacctt ttgggcctcg gccacttcct ccaccctttg 1141 gccctggtat gcgtccacca ctaggcttaa gagaatttgc accaggcgtt ccaccaggaa 1201 gacgggacct gcctctccac cctcggggat ttttacctgg acacgcacca tttagacctt 1261 taggttcact tggcccaaga gagtacttta ttcctggtac ccgattacca cccccaaccc 1321 atggtcccca ggaataccca ccaccacctg ctgtaagaga cttactgccg tcaggctcta 1381 gagatgagcc tccacctgcc tctcagagca ctagccagga ctgttcacag gctttaaaac 1441 agagcccata cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1501 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1561 tcaacctctg gatta