Construct: ORF ccsbBroad304_13689

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017734.1_s304c1, BRDN0000408002
Derived from:
ccsbBroadEn_13689
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF2AK4 (440275)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13689
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440275 EIF2AK4 eukaryotic translation init... NM_001013703.4 37.1% 36.9% (many diffs)
2 mouse 27103 Eif2ak4 eukaryotic translation init... NM_013719.3 31.9% 32.8% (many diffs)
3 mouse 27103 Eif2ak4 eukaryotic translation init... XM_017318909.1 30.4% 31.2% (many diffs)
4 mouse 27103 Eif2ak4 eukaryotic translation init... NM_001177806.1 26.1% 26.7% (many diffs)
5 mouse 27103 Eif2ak4 eukaryotic translation init... XM_006499625.3 26.1% 26.7% (many diffs)
6 mouse 27103 Eif2ak4 eukaryotic translation init... XM_006499626.3 25.3% 26% (many diffs)
7 mouse 27103 Eif2ak4 eukaryotic translation init... XM_006499627.3 23.8% 24.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1914
ORF length:
1848
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tgggggccgt ggggcccccg ggcgcggccg ggacgagcct ccggagagct
121acccgcaacg acaggaccac gagctacagg ccctggaggc catctacggc gcggacttcc
181aagacctgcg gccggacgct tgcggaccgg tcaaagagcc ccctgaaatc aatttagttt
241tgtaccctca aggcctaact ggtgaagaag tatatgtaaa agtggatttg agggttaaat
301gcccacctac ctatccagat gtagttcctg aaatagagtt aaaaaatgcc aaaggtctat
361caaatgaaag tgtcaatttg ttaaaatctc gcctagaaga actggccaag aaacactgtg
421gggaggtgat gatctttgaa ctggcttacc acgtgcagtc atttctcagc gagcataaca
481agccccctcc caagtctttt catgaagaaa tgctggaaag gcgggctcag gaggagcagc
541agaggctgtt ggaggccaag cggaaagaag agcaggagca acgtgaaatc ctgcatgaga
601ttcagagaag gaaagaagag ataaaagaag agaaaaaaag gaaagaaatg gctaagcagg
661aacgtttgga aattgctagt ttgtcaaacc aagatcatac ctctaagaag gacccaggag
721gacacagaac ggctgccatt ctacatggag gctctcctga ctttgtagga aatggtaaac
781atcgggcaaa ctcctcagga aggtctaggc gagaacgtca gtattctgta tgtaatagtg
841aagattctcc tggctcttgt gaaattctgt atttcaatat ggggagtcct gatcagctca
901tggtgcacaa agggaaatgt attggcagtg atgaacaact tggaaaatta gtctacaatg
961ctttggaaac agccactggt ggctttgtct tgttgtatga gtgggtcctt cagtggcaga
1021aaaaaatggg tccattcctt accagtcaag aaaaagagaa gattgataag tgcaaaaagc
1081agattcaagg aacagaaaca gaattcaact cactggtaaa attgagccat ccaaatgtag
1141tacgctacct tgcaatgaat ctcaaagagc aagacgactc catcgtggtg gacattttag
1201tggagcacat tagtggggtc tctcttgctg cacacctgag ccactcaggc cccatccctg
1261tgcatcagct tcgcaggtac acagctcagc tcctgtcagg ccttgattat ctgcacagca
1321attctgtggt gcataaggtc ctgagtgcat ctaatgtctt ggtggatgca gaaggcaccg
1381tcaagattac ggactatagc atttctaagc gcctcgcaga catttgcaag gaggatgtgt
1441ttgagcaaac ccgagttcgt tttagtgaca atgctctgcc ttataaaacg gggaagaaag
1501gagatgtttg gcgtcttggc cttctgctgc tgtccctcag ccaaggacag gaatgtggag
1561agtaccctgt gaccatccct agtgacttac cagctgactt tcaagatttt ctaaagaaat
1621gtgtgtgctt ggatgacaag gaaagatgga gtccccagca gttgttgaaa cacagcttta
1681taaatcccca gccaaaaatg cctctagtgg aacaaagtcc tgaagattct gaaggacaag
1741attatgttga gactgttatt cctagcaacc ggctacccag tgctgccttc tttagtgaga
1801cacagagaca gttttcccga tacttcattg agtttgaaga attacaactt cttggtaaag
1861gagcttttgg agctgtcatc aaggtgtggt acagagtcat tcccagtccc ctttacccaa
1921ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1981cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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