Transcript: Mouse XM_006499626.3

PREDICTED: Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Eif2ak4 (27103)
Length:
4879
CDS:
128..4705

Additional Resources:

NCBI RefSeq record:
XM_006499626.3
NBCI Gene record:
Eif2ak4 (27103)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006499626.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361611 CAAACTCGTCTGCGATGAAAT pLKO_005 4603 CDS 100% 13.200 18.480 N Eif2ak4 n/a
2 TRCN0000361612 GTTGGTGAAGTATAGCTTAAA pLKO_005 3613 CDS 100% 13.200 18.480 N Eif2ak4 n/a
3 TRCN0000028778 CCTCGGAAGTTAGACCGATTT pLKO.1 3188 CDS 100% 10.800 15.120 N Eif2ak4 n/a
4 TRCN0000028830 CGGTACTTCATTGAGTTTGAA pLKO.1 1508 CDS 100% 5.625 7.875 N Eif2ak4 n/a
5 TRCN0000235995 TCTGGATGGATTAGCTTATAT pLKO_005 2248 CDS 100% 15.000 12.000 N Eif2ak4 n/a
6 TRCN0000235996 GACTACTACAGAATCCTATTT pLKO_005 4682 CDS 100% 13.200 9.240 N Eif2ak4 n/a
7 TRCN0000028842 GCCAACCATCAACTCACTAAT pLKO.1 3568 CDS 100% 13.200 9.240 N Eif2ak4 n/a
8 TRCN0000028779 CGAGAGATTCTGGATGGATTA pLKO.1 2240 CDS 100% 10.800 7.560 N Eif2ak4 n/a
9 TRCN0000235994 CTAAAGAACTGCTGCTGTATT pLKO_005 4708 3UTR 100% 13.200 7.920 N Eif2ak4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006499626.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15327 pDONR223 0% 46.7% 49.3% None (many diffs) n/a
2 ccsbBroad304_15327 pLX_304 0% 46.7% 49.3% V5 (many diffs) n/a
3 TRCN0000468897 TAATAACTGCTATCGATAGTCCCA pLX_317 16.1% 46.7% 49.3% V5 (many diffs) n/a
4 ccsbBroadEn_14503 pDONR223 100% 46.7% 49.2% None (many diffs) n/a
5 ccsbBroad304_14503 pLX_304 0% 46.7% 49.2% V5 (many diffs) n/a
6 TRCN0000473863 TAATGGTTTCACAGGGAACGAGCC pLX_317 23% 46.7% 49.2% V5 (many diffs) n/a
7 ccsbBroadEn_13689 pDONR223 100% 25.3% 26% None (many diffs) n/a
8 ccsbBroad304_13689 pLX_304 0% 25.3% 26% V5 (many diffs) n/a
9 TRCN0000470577 TGGACCGTGATACTAATAGAACGA pLX_317 23.1% 25.3% 26% V5 (many diffs) n/a
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