Construct: ORF ccsbBroad304_13715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000531.1_s304c1, BRDN0000404604
- Derived from:
- ccsbBroadEn_13715
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC554223 (554223)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13715
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010817.1 | 37.4% | 30.4% | (many diffs) |
| 2 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010818.1 | 37.4% | 30.4% | (many diffs) |
| 3 | human | 3135 | HLA-G | major histocompatibility co... | NM_002127.5 | 26.9% | 22.1% | (many diffs) |
| 4 | human | 3135 | HLA-G | major histocompatibility co... | XM_024446420.1 | 26.9% | 22.1% | (many diffs) |
| 5 | human | 3135 | HLA-G | major histocompatibility co... | NM_001363567.1 | 26.5% | 21.8% | (many diffs) |
| 6 | human | 3105 | HLA-A | major histocompatibility co... | NM_002116.8 | 25.1% | 21% | (many diffs) |
| 7 | human | 3105 | HLA-A | major histocompatibility co... | NM_001242758.1 | 24.9% | 20.8% | (many diffs) |
| 8 | human | 352962 | HLA-V | major histocompatibility co... | NR_132323.1 | 24.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 384
- ORF length:
- 318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gccccgaacc ctgcttctgc tgctctcggg ggccctggtc ctgacccaga 121 cctgggcagg cttccactcc ttgaggtatt tccacaccac catgtcccgg cccggccgcg 181 cggatccccg cttcctctcc gtgggcgacg tggacgacac gcagtgcgtg cggctcgaca 241 gcgacgccac gagtcccagg atggagccgg aggggccgga atattgggaa gaggagacag 301 ggaccgccaa ggccaaagca cagttttacc gagtgaacct gcggaccctg agcggctact 361 acaaccagag tgaggcctgt gagtgcccaa ctttcttgta caaagtggtt ggtaagccta 421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 481 gcgttaagtc gacaatcaac ctctggatta