Construct: ORF ccsbBroad304_13726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002899.1_s304c1, BRDN0000460203
- Derived from:
- ccsbBroadEn_13726
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS18P9 (645958)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13726
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6222 | RPS18 | ribosomal protein S18 | NM_022551.3 | 46.4% | 45.3% | (many diffs) |
| 2 | human | 645958 | RPS18P9 | ribosomal protein S18 pseud... | NR_077247.1 | 22.2% | 1_703del;903T>C;926_993del | |
| 3 | mouse | 20084 | Rps18 | ribosomal protein S18 | NM_011296.2 | 41.4% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 291
- ORF length:
- 222
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccagcctgg ttcttgaaca gacagaagga tgtaaaggat ggaaaatata 121 gccaggtcct agccaatggt ctggacaaca agctctgtga agacctggag cgactgaaaa 181 agattcgggc ccgtagaggg ctgcgtcact tctggggcct tcgtgtccga ggccagcaca 241 ccaagaccac aggctgccgt ggccgcaccg tgggtgtgtc taagaagaag ttgccaactt 301 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 361 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta