Construct: ORF ccsbBroad304_13868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001780.1_s304c1, BRDN0000398892
- Derived from:
- ccsbBroadEn_13868
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GYG1 (2992)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13868
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2992 | GYG1 | glycogenin 1 | NM_001184720.1 | 99.8% | 14delC | |
| 2 | human | 2992 | GYG1 | glycogenin 1 | NM_004130.3 | 95% | 14delC;828_878del | |
| 3 | human | 2992 | GYG1 | glycogenin 1 | XM_017006275.1 | 82.3% | 0_1ins176 | |
| 4 | human | 2992 | GYG1 | glycogenin 1 | NM_001184721.1 | 73.6% | 14delC;608_609ins220;780_837del | |
| 5 | human | 2992 | GYG1 | glycogenin 1 | XM_017006276.1 | 52.9% | (many diffs) | |
| 6 | mouse | 27357 | Gyg | glycogenin | NM_013755.3 | 87.2% | (many diffs) | |
| 7 | mouse | 27357 | Gyg | glycogenin | XM_006535482.3 | 74.2% | (many diffs) | |
| 8 | mouse | 27357 | Gyg | glycogenin | XM_006535481.2 | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 84
- ORF length:
- 18
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac agatcaggct ttgtgacact aaccacaaac gatgcctacg ccaaaggtgc 121 cctggtcctg ggatcatctc tgaaacagca caggaccacc aggaggctgg tcgtgctcgc 181 cacccctcag gtctcagact ccatgagaaa agttttagag acagtctttg atgaagtcat 241 catggtagat gtcttggaca gtggcgattc tgctcatcta accttaatga agaggccaga 301 gttgggtgtc acgctgacaa agctccactg ctggtcgctt acacagtatt caaaatgtgt 361 attcatggat gcagatactc tggtcctagc aaatattgat gatctttttg acagagaaga 421 attgtcagca gcaccagacc cagggtggcc tgactgcttc aattccggag tcttcgttta 481 tcagccttca gttgaaacat acaatcagct gttgcatctt gcttctgagc aaggtagttt 541 tgatggtggg gaccaaggca tactgaacac attttttagc agctgggcaa caacagatat 601 cagaaaacac ctgccgttta tttataacct aagcagcatc tctatatact cctacctccc 661 ggcatttaaa gtgtttggtg caagtgccaa agttgtgcat ttcctgggac gagtcaaacc 721 atggaattat acttatgatc ccaaaacaaa aagtgtcaaa agtgaggccc atgatcccaa 781 catgactcat ccagagtttc tcatcctgtg gtggaacatc tttaccacca acgttttacc 841 tctgcttcaa caatttggcc ttgtcaaaga cacctgctca tatgtaaatg tggaagatgt 901 ctcaggagcc atatcacatc tgtcccttgg ggagatccca gctatggcac agccgtttgt 961 atcctcggaa gaacggaagg aacgatggga acagggccag gctgattata tgggagcaga 1021 ttcctttgac aacatcaaga ggaaacttga cacttacctc cagtacccaa ctttcttgta 1081 caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg 1141 agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta