Construct: ORF ccsbBroad304_13886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017800.1_s304c1, BRDN0000407438
- Derived from:
- ccsbBroadEn_13886
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNMB1 (3779)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13886
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3779 | KCNMB1 | potassium calcium-activated... | NM_004137.4 | 62.6% | 49.2% | (many diffs) |
| 2 | mouse | 16533 | Kcnmb1 | potassium large conductance... | NM_031169.4 | 54.5% | 44.1% | (many diffs) |
| 3 | mouse | 16533 | Kcnmb1 | potassium large conductance... | XM_006514541.3 | 54.5% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gaagaagctg gtgatggccc agaagcgggg agagacacga gccctttgcc 121 tgggtgtaac catggtggtg tgtgccgtca tcacctacta catcctggtc acgactgtgc 181 tgcccctcta ccagaaaagc gtgtggaccc aggaatccaa gtgccacctg attgagacca 241 acatcaggga ccaggaggag ctgaagggca agaaggtgcc ccagtaccca tgcctgtggg 301 tcaacgtgtc agctgccggc aggtgggctg tgctgtacca cacggaggac actcgggacc 361 agaaccagca ggtactgaac tggagggatg gggacacatc cctttatccc tgtcaggtgt 421 gtgagcctgt tcctaactgt ccctgtcccc caggctgccc aactttcttg tacaaagtgg 481 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 541 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta