Transcript: Human NM_004137.4

Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
KCNMB1 (3779)
Length:
4742
CDS:
134..709

Additional Resources:

NCBI RefSeq record:
NM_004137.4
NBCI Gene record:
KCNMB1 (3779)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004137.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000043794 GAACGAAACCAGCGTCCTATT pLKO.1 556 CDS 100% 10.800 15.120 N KCNMB1 n/a
2 TRCN0000043793 CCACCTGATTGAGACCAACAT pLKO.1 292 CDS 100% 4.950 6.930 N KCNMB1 n/a
3 TRCN0000444470 GTTTCCGGCTGCATCACTATG pLKO_005 988 3UTR 100% 10.800 7.560 N KCNMB1 n/a
4 TRCN0000043796 GAATCCAAGTGCCACCTGATT pLKO.1 281 CDS 100% 4.950 3.465 N KCNMB1 n/a
5 TRCN0000043795 CATGGTGAAGAGCAACCAGTA pLKO.1 661 CDS 100% 4.050 2.835 N KCNMB1 n/a
6 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2970 3UTR 100% 4.950 2.475 Y CFLAR n/a
7 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2970 3UTR 100% 4.950 2.475 Y C19orf31 n/a
8 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 1616 3UTR 100% 4.050 2.025 Y INTS7 n/a
9 TRCN0000043797 GAGCCCTTTGCCTGGGTGTAA pLKO.1 177 CDS 100% 1.650 0.825 Y KCNMB1 n/a
10 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2968 3UTR 100% 4.950 2.475 Y ERN2 n/a
11 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2968 3UTR 100% 4.950 2.475 Y P3H4 n/a
12 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2968 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004137.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13886 pDONR223 100% 62.6% 49.2% None (many diffs) n/a
2 ccsbBroad304_13886 pLX_304 0% 62.6% 49.2% V5 (many diffs) n/a
3 TRCN0000480872 TTGTGGGAATCAAATTGGTGGATC pLX_317 100% 62.6% 49.2% V5 (many diffs) n/a
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