Construct: ORF ccsbBroad304_13918

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014773.1_s304c1, BRDN0000462200
Derived from:
ccsbBroadEn_13918
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ENPP1 (5167)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13918
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5167 ENPP1 ectonucleotide pyrophosphat... NM_006208.3 94.3% 36.2% 1_156del;1162G>T;2762T>G
2 mouse 18605 Enpp1 ectonucleotide pyrophosphat... NM_008813.4 78.4% 32.5% (many diffs)
3 mouse 18605 Enpp1 ectonucleotide pyrophosphat... NM_001308327.1 78.4% 32.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1074
ORF length:
1005
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggacgtgggg gaggagccgc tggagaaggc ggcgcgcgcc cgcactgcca
121aggaccccaa cacctataaa gtactctcgc tggtattgtc agtatgtgtg ttaacaacaa
181tacttggttg tatatttggg ttgaaaccaa gctgtgccaa agaagttaaa agttgcaaag
241gtcgctgttt cgagagaaca tttgggaact gtcgctgtga tgctgcctgt gttgagcttg
301gaaactgctg tttagattac caggagacgt gcatagaacc agaacatata tggacttgca
361acaaattcag gtgtggtgag aaaaggttga ccagaagcct ctgtgcctgt tcagatgact
421gcaaggacaa gggcgactgc tgcatcaact acagttctgt gtgtcaaggt gagaaaagtt
481gggtagaaga accatgtgag agcattaatg agccacagtg cccagcaggg tttgaaacgc
541ctcctaccct cttattttct ttggatggat tcagggcaga atatttacac acttggggtg
601gacttcttcc tgttattagc aaactaaaaa aatgtggaac atatactaaa aacatgagac
661cggtatatcc aacaaaaact ttccccaatc actacagcat tgtcaccgga ttgtatccag
721aatctcatgg cataatcgac aataaaatgt atgatcccaa aatgaatgct tccttttcac
781ttaaaagtaa agagaaattt aatcctgagt ggtacaaagg agaaccaatt tgggtcacag
841ctaagtatca aggcctcaag tctggcacat ttttctggcc aggatcagat gtggaaatta
901acggaatttt cccagacatc tataaaatgt ataatggttc agtaccattt gaagaaagga
961ttttagctgt tcttcagtgg ctacagcttc ctaaagatga aagaccacac ttttacactc
1021tgtatttaga agaaccagat tcttcaggtc attcatatgg accagtcagc agttaagtca
1081tcaaagcctt gcagagggtt gatggtatgg ttggtatgct gatggatggt ctgaaagagc
1141tgaacttgca cagatgcctg aacctcatcc ttatttcaga tcatggcatg gaacaaggca
1201gttgtaagaa atacatatat ctgaataaat atttggggga tgttaaaaat attaaagtta
1261tctatggacc tgcagctcga ttgagaccct ctgatgtccc agataaatac tattcattta
1321actatgaagg cattgcccga aatctttctt gccgggaacc aaaccagcac ttcaaacctt
1381acctgaaaca tttcttacct aagcgtttgc actttgctaa gagtgataga attgagccct
1441tgacattcta tttggaccct cagtggcaac ttgcattgaa tccctcagaa aggaaatatt
1501gtggaagtgg atttcatggc tctgacaatg tattttcaaa tatgcaagcc ctctttgttg
1561gctatggacc tggattcaag catggcattg aggctgacac ctttgaaaac attgaagtct
1621ataacttaat gtgtgattta ctgaatttga caccggctcc taataacgga actcatggaa
1681gtcttaacca ccttctaaag aatcctgttt atacgccaaa gcatcccaaa gaagtgcacc
1741ccctggtaca gtgccccttc acaagaaacc ccagagataa ccttggctgc tcatgtaacc
1801cttcgatttt gccgattgag gattttcaaa cacagttcaa tctgactgtg gcagaagaga
1861agattattaa gcatgaaact ttaccctatg gaagacctag agttctccag aaggaaaaca
1921ccatctgtct tctttcccag caccagttta tgagtggata cagccaagac atcttaatgc
1981ccctttggac atcctatacc gtggacagaa atgacagttt ctctacggaa gacttctcca
2041actgtctgta ccaggacttt agaattcctc ttagtcctgt ccataaatgt tcattttata
2101aaaataacac caaagtgagt tacgggttcc tctccccacc acaactaaat aaaaattcaa
2161gtggaatata ttctgaagct ttgcttacta caaatatagt gccaatgtac cagagttttc
2221aagttatatg gcgctacttt catgacaccc tactgcgaaa gtatgctgaa gaaagaaatg
2281gtgtcaatgt cgtcagtggt cctgtgtttg actttgatta tgatggacgt tgtgattcct
2341tagagaatct gaggcaaaaa agaagagtca tccgtaacca agaaattttg attccaactc
2401acttctttat tgtgctaaca agctgtaaag atacatctca gacgcctttg cactgtgaaa
2461acctagacac cttagctttc attttgcctc acaggactga taacagcgag agctgtgtgc
2521atgggaagca tgactcctca tgggttgaag aattgttaat gttacacaga gcacggatca
2581cagatgttga gcacatcact ggactcagct tctatcaaca aagaaaagag ccagtttcag
2641acattttaaa gttgaaaaca catttgccaa cctgtagcca agaagacttg ccaactttct
2701tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2761aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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