Construct: ORF ccsbBroad304_13925

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007780.1_s304c1, BRDN0000409637
Derived from:
ccsbBroadEn_13925
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PPP2R1B (5519)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13925
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5519 PPP2R1B protein phosphatase 2 scaff... NM_181699.3 99.9% .5% 3delG;936A>T
2 human 5519 PPP2R1B protein phosphatase 2 scaff... XM_024448598.1 96.5% 1.2% (many diffs)
3 human 5519 PPP2R1B protein phosphatase 2 scaff... XM_024448599.1 95.7% 1.2% (many diffs)
4 human 5519 PPP2R1B protein phosphatase 2 scaff... XM_017017960.2 93.1% .6% 3delG;936A>T;1027_1028ins135
5 human 5519 PPP2R1B protein phosphatase 2 scaff... XM_017017961.1 91.2% .4% (many diffs)
6 human 5519 PPP2R1B protein phosphatase 2 scaff... NM_181700.2 90.3% .6% 3delG;113_114ins192;744A>T
7 human 5519 PPP2R1B protein phosphatase 2 scaff... NM_002716.5 89.4% 1.3% (many diffs)
8 human 5519 PPP2R1B protein phosphatase 2 scaff... XM_024448600.1 89% 1.3% (many diffs)
9 human 5519 PPP2R1B protein phosphatase 2 scaff... NM_001177562.2 82.7% 1.4% (many diffs)
10 human 5519 PPP2R1B protein phosphatase 2 scaff... NM_001177563.2 70.4% 1.6% (many diffs)
11 mouse 73699 Ppp2r1b protein phosphatase 2, regu... NM_001034085.2 85.9% 1.2% (many diffs)
12 mouse 73699 Ppp2r1b protein phosphatase 2, regu... NM_001286553.1 84.4% 1% (many diffs)
13 mouse 73699 Ppp2r1b protein phosphatase 2, regu... NM_028614.3 78.9% 1.3% (many diffs)
14 mouse 73699 Ppp2r1b protein phosphatase 2, regu... XM_006510641.3 73.5% 1% (many diffs)
15 mouse 73699 Ppp2r1b protein phosphatase 2, regu... XM_006510642.3 70.8% 1% (many diffs)
16 mouse 73699 Ppp2r1b protein phosphatase 2, regu... XR_001779024.1 46.3% (many diffs)
17 mouse 73699 Ppp2r1b protein phosphatase 2, regu... XR_001779023.1 46.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
147
ORF length:
81
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg ggcgcatcag agctcgggac cggcccagga gcagcgggtg gagatggaga
121tgattcgcta tacccgatcg cggttttaat cgacgagctc cgcaatgaag acgtgcagct
181ccgactcaac agtattaaga agttatcaac aattgcccta gcacttggag tagaaaggac
241ccgaagtgaa ttgttgccat ttcttacaga tacaatttat gatgaagatg aggtactatt
301agctcttgct gagcagctgg gaaatttcac tggcctagtg ggaggtcctg actttgccca
361ctgtctgctg cctcctttgg aaaatctggc aactgtggaa gagactgttg ttcgtgacaa
421ggctgtggag tccctgagac agatctccca ggagcatact cctgttgctc tggaagctta
481ttttgtacct ctggtgaaac gcttagcaag tggggattgg ttcacctctc gcacatctgc
541atgtggtttg ttcagcgttt gctatcccag ggcatcaaat gctgttaaag cagaaatcag
601acagcaattc cgttccttgt gctcagatga cacaccaatg gtacgacgtg ctgctgcttc
661caaattgggt gaatttgcaa aagttttgga attagacagt gtgaaaagtg aaattgttcc
721actgttcact agtctagctt cagatgaaca ggattcagtg cgcctccttg ctgtggaagc
781ttgtgtcagt attgcccagt tattgtctca ggatgacctt gagactttgg tgatgcctac
841acttcgacaa gcagcagaag ataaatcttg gcgcgttcgc tatatggtgg ctgacagatt
901ttcagagctc cagaaagcca tgggtcctaa aatcacccta aatgacctca tccccgcctt
961tcagaaccta cttaaagact gtgaagctga agtccgggct gctgctgccc acaaagtaaa
1021agaacttggt gagaacttgc ccattgaaga tagagagacc ataattatga atcaaattct
1081gccttatata aaggaattag tatccgatac caatcaacat gtcaaatcgg ctctagcttc
1141tgtaattatg ggattgtcta ctattttggg caaagaaaat accattgaac atcttctacc
1201tcttttctta gctcagttaa aggatgagtg tcctgacgtt cgtttgaata tcatctccaa
1261tttggattgt gtaaatgaag tgattggaat ccgtcagctc tctcagtctc tccttcctgc
1321catagtggag ctggcagaag atgccaaatg gagggtccgc ctggccatca ttgagtatat
1381gccgctgctg gcaggccagc tgggtgtgga attctttgat gaaaagctga attctttatg
1441tatggcttgg ctcgtggacc atgtatacgc catccgagaa gctgccacca acaacctcat
1501gaaactagtt cagaagtttg gtacagagtg ggcccaaaat actattgttc ccaaagtgtt
1561agtaatggca aatgatccta attacttgca tagaatgacc actttattct gcattaatgc
1621actgtctgag gcctgtggtc aggaaataac tactaagcaa atgctgccca tcgtattaaa
1681aatggcagga gaccaagtag caaatgttcg cttcaatgtg gccaaatctc tacaaaagat
1741tggaccaatt ctagatacca atgctttaca gggagaagtg aagccagtac tacagaagtt
1801aggtcaagat gaagacatgg atgtcaaata ctttgcacag gaagctataa gtgtggtggc
1861acaaaggctg aggaagctag aatttcctgt gaaggacagt ggagaaccca gtgtccctcg
1921ggctgacaag aaccacttcc caagacccac agtgcctgga gaggacatgg ggaagggacc
1981agtgtatcag ttgcgtggag atactagaga cacacttgcc cagctgggaa ttgcagagct
2041agtgcatttc tcccaaagca cagactaccc aactttcttg tacaaagtgg ttggtaagcc
2101tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg
2161gcgcgttaag tcgacaatca acctctggat ta

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