Construct: ORF ccsbBroad304_13925
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007780.1_s304c1, BRDN0000409637
- Derived from:
- ccsbBroadEn_13925
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP2R1B (5519)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13925
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | NM_181699.3 | 99.9% | .5% | 3delG;936A>T |
| 2 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | XM_024448598.1 | 96.5% | 1.2% | (many diffs) |
| 3 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | XM_024448599.1 | 95.7% | 1.2% | (many diffs) |
| 4 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | XM_017017960.2 | 93.1% | .6% | 3delG;936A>T;1027_1028ins135 |
| 5 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | XM_017017961.1 | 91.2% | .4% | (many diffs) |
| 6 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | NM_181700.2 | 90.3% | .6% | 3delG;113_114ins192;744A>T |
| 7 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | NM_002716.5 | 89.4% | 1.3% | (many diffs) |
| 8 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | XM_024448600.1 | 89% | 1.3% | (many diffs) |
| 9 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | NM_001177562.2 | 82.7% | 1.4% | (many diffs) |
| 10 | human | 5519 | PPP2R1B | protein phosphatase 2 scaff... | NM_001177563.2 | 70.4% | 1.6% | (many diffs) |
| 11 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | NM_001034085.2 | 85.9% | 1.2% | (many diffs) |
| 12 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | NM_001286553.1 | 84.4% | 1% | (many diffs) |
| 13 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | NM_028614.3 | 78.9% | 1.3% | (many diffs) |
| 14 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | XM_006510641.3 | 73.5% | 1% | (many diffs) |
| 15 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | XM_006510642.3 | 70.8% | 1% | (many diffs) |
| 16 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | XR_001779024.1 | 46.3% | (many diffs) | |
| 17 | mouse | 73699 | Ppp2r1b | protein phosphatase 2, regu... | XR_001779023.1 | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 147
- ORF length:
- 81
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg ggcgcatcag agctcgggac cggcccagga gcagcgggtg gagatggaga 121 tgattcgcta tacccgatcg cggttttaat cgacgagctc cgcaatgaag acgtgcagct 181 ccgactcaac agtattaaga agttatcaac aattgcccta gcacttggag tagaaaggac 241 ccgaagtgaa ttgttgccat ttcttacaga tacaatttat gatgaagatg aggtactatt 301 agctcttgct gagcagctgg gaaatttcac tggcctagtg ggaggtcctg actttgccca 361 ctgtctgctg cctcctttgg aaaatctggc aactgtggaa gagactgttg ttcgtgacaa 421 ggctgtggag tccctgagac agatctccca ggagcatact cctgttgctc tggaagctta 481 ttttgtacct ctggtgaaac gcttagcaag tggggattgg ttcacctctc gcacatctgc 541 atgtggtttg ttcagcgttt gctatcccag ggcatcaaat gctgttaaag cagaaatcag 601 acagcaattc cgttccttgt gctcagatga cacaccaatg gtacgacgtg ctgctgcttc 661 caaattgggt gaatttgcaa aagttttgga attagacagt gtgaaaagtg aaattgttcc 721 actgttcact agtctagctt cagatgaaca ggattcagtg cgcctccttg ctgtggaagc 781 ttgtgtcagt attgcccagt tattgtctca ggatgacctt gagactttgg tgatgcctac 841 acttcgacaa gcagcagaag ataaatcttg gcgcgttcgc tatatggtgg ctgacagatt 901 ttcagagctc cagaaagcca tgggtcctaa aatcacccta aatgacctca tccccgcctt 961 tcagaaccta cttaaagact gtgaagctga agtccgggct gctgctgccc acaaagtaaa 1021 agaacttggt gagaacttgc ccattgaaga tagagagacc ataattatga atcaaattct 1081 gccttatata aaggaattag tatccgatac caatcaacat gtcaaatcgg ctctagcttc 1141 tgtaattatg ggattgtcta ctattttggg caaagaaaat accattgaac atcttctacc 1201 tcttttctta gctcagttaa aggatgagtg tcctgacgtt cgtttgaata tcatctccaa 1261 tttggattgt gtaaatgaag tgattggaat ccgtcagctc tctcagtctc tccttcctgc 1321 catagtggag ctggcagaag atgccaaatg gagggtccgc ctggccatca ttgagtatat 1381 gccgctgctg gcaggccagc tgggtgtgga attctttgat gaaaagctga attctttatg 1441 tatggcttgg ctcgtggacc atgtatacgc catccgagaa gctgccacca acaacctcat 1501 gaaactagtt cagaagtttg gtacagagtg ggcccaaaat actattgttc ccaaagtgtt 1561 agtaatggca aatgatccta attacttgca tagaatgacc actttattct gcattaatgc 1621 actgtctgag gcctgtggtc aggaaataac tactaagcaa atgctgccca tcgtattaaa 1681 aatggcagga gaccaagtag caaatgttcg cttcaatgtg gccaaatctc tacaaaagat 1741 tggaccaatt ctagatacca atgctttaca gggagaagtg aagccagtac tacagaagtt 1801 aggtcaagat gaagacatgg atgtcaaata ctttgcacag gaagctataa gtgtggtggc 1861 acaaaggctg aggaagctag aatttcctgt gaaggacagt ggagaaccca gtgtccctcg 1921 ggctgacaag aaccacttcc caagacccac agtgcctgga gaggacatgg ggaagggacc 1981 agtgtatcag ttgcgtggag atactagaga cacacttgcc cagctgggaa ttgcagagct 2041 agtgcatttc tcccaaagca cagactaccc aactttcttg tacaaagtgg ttggtaagcc 2101 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg 2161 gcgcgttaag tcgacaatca acctctggat ta