Construct: ORF ccsbBroad304_13929

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010311.1_s304c1, BRDN0000461135
Derived from:
ccsbBroadEn_13929
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRKACA (5566)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13929
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5566 PRKACA protein kinase cAMP-activat... NM_002730.4 99.9% 1.7% 8_9insC
2 human 5566 PRKACA protein kinase cAMP-activat... NM_207518.3 96.6% .5% (many diffs)
3 human 5568 PRKACG protein kinase cAMP-activat... NM_002732.3 85.3% 1.4% (many diffs)
4 human 5566 PRKACA protein kinase cAMP-activat... XM_017026948.1 84.2% 2% 8_9insC;760_761ins165
5 human 5566 PRKACA protein kinase cAMP-activat... NM_001304349.1 81.6% 1.1% (many diffs)
6 mouse 18747 Prkaca protein kinase, cAMP depend... NM_008854.5 91.1% 1.7% (many diffs)
7 mouse 18747 Prkaca protein kinase, cAMP depend... NM_001277898.1 88.3% 2.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
153
ORF length:
84
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggcaaccgc cgccgccgcc aagaagggca gcgagcagga gagcgtgaaa
121gaattcttag ccaaagccaa agaagatttt cttaaaaaat gggaaagtcc cgctcagaac
181acagcccact tggatcagtt tgaacgaatc aagaccctcg gcacgggctc cttcgggcgg
241gtgatgctgg tgaaacacaa ggagaccggg aaccactatg ccatgaagat cctcgacaaa
301cagaaggtgg tgaaactgaa acagatcgaa cacaccctga atgaaaagcg catcctgcaa
361gctgtcaact ttccgttcct cgtcaaactc gagttctcct tcaaggacaa ctcaaactta
421tacatggtca tggagtacgt gcccggcggg gagatgttct cacacctacg gcggatcgga
481aggttcagtg agccccatgc ccgtttctac gcggcccaga tcgtcctgac ctttgagtat
541ctgcactcgc tggatctcat ctacagggac ctgaagccgg agaatctgct cattgaccag
601cagggctaca ttcaggtgac agacttcggt ttcgccaagc gcgtgaaggg ccgcacttgg
661accttgtgcg gcacccctga gtacctggcc cctgagatta tcctgagcaa aggctacaac
721aaggccgtgg actggtgggc cctgggggtt cttatctatg aaatggccgc tggctacccg
781cccttcttcg cagaccagcc catccagatc tatgagaaga tcgtctctgg gaaggtgcgc
841ttcccttccc acttcagctc tgacttgaag gacctgctgc ggaacctcct gcaggtagat
901ctcaccaagc gctttgggaa cctcaagaat ggggtcaacg atatcaagaa ccacaagtgg
961tttgccacaa ctgactggat tgccatctac cagaggaagg tggaagctcc cttcatacca
1021aagtttaaag gccctgggga tacgagtaac tttgacgact atgaggaaga agaaatccgg
1081gtctccatca atgagaagtg tggcaaggag ttttctgagt ttttgccaac tttcttgtac
1141aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
1201gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)