Construct: ORF ccsbBroad304_13931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014118.1_s304c1, BRDN0000404122
- Derived from:
- ccsbBroadEn_13931
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PSMA3 (5684)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13931
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5684 | PSMA3 | proteasome 20S subunit alpha 3 | NM_152132.2 | 99.4% | 53.3% | 401_402insNNNN |
| 2 | human | 5684 | PSMA3 | proteasome 20S subunit alpha 3 | NM_002788.4 | 97.2% | 51.9% | 402_422delinsNNNN |
| 3 | human | 5684 | PSMA3 | proteasome 20S subunit alpha 3 | NR_038123.1 | 72.7% | (many diffs) | |
| 4 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | NM_011184.5 | 89.4% | 51.9% | (many diffs) |
| 5 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | XM_017314996.1 | 89.4% | 51.9% | (many diffs) |
| 6 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | NM_001310595.1 | 62% | 22.8% | (many diffs) |
| 7 | mouse | 19167 | Psma3 | proteasome (prosome, macrop... | NM_001310596.1 | 62% | 22.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcaatcggc actgggtatg acctgtcagc ctctacattc tctcctgacg 121 gaagagtttt tcaagttgaa tatgctatga aggctgtgga aaatagtagt acagctattg 181 gaatcagatg caaagatggt gttgtctttg gggtagaaaa attagtcctt tctaaacttt 241 atgaagaagg ttccaacaaa agacttttta atgttgatcg gcatgttgga atggcagtag 301 caggtttgtt ggcagatgct cgttctttag cagacatagc aagagaagaa gcttccaact 361 tcagatctaa ctttggctac aacattccac taaaacatct tgcagacaga gtggccatgt 421 atgtgcatgc atatacactc tacagtgctg ttagaccttt tggctgnnnn cagtgtgaat 481 gacggtgcgc aactctacat gattgaccca tcaggtgttt catacggtta ttggggctgt 541 gccatcggca aagccaggca agctgcaaag acggaaatag agaagcttca gatgaaagaa 601 atgacctgcc gtgatatcgt taaagaagtt gcaaaaataa tttacatagt acatgacgaa 661 gttaaggata aagcttttga actagaactc agctgggttg gtgaattaac taatggaaga 721 catgaaattg ttccaaaaga tataagagaa gaagcagaga aatatgctaa ggaatctctg 781 aaggaagaag atgaatcaga tgatgataat atgtacccaa ctttcttgta caaagtggtt 841 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct 901 aaccggtggc gcgttaagtc gacaatcaac ctctggatta