Construct: ORF ccsbBroad304_13935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008867.1_s304c1, BRDN0000408333
- Derived from:
- ccsbBroadEn_13935
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPN3 (5774)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13935
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NM_002829.4 | 99.9% | 99.8% | 2736delT |
| 2 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_006717199.3 | 99.9% | 99.8% | 2736delT |
| 3 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_011518889.2 | 99.9% | 99.8% | 2736delT |
| 4 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_017014955.1 | 99.9% | 99.8% | 2736delT |
| 5 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_006717197.3 | 98.7% | 98.7% | 1_33del;2769delT |
| 6 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_011518888.2 | 97.7% | 97.6% | 1_63del;2799delT |
| 7 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NM_001145368.2 | 95% | 94.9% | 1001_1002ins135;2601delT |
| 8 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_006717201.3 | 88% | 86.6% | 0_1ins248;39_40ins79;2409delT |
| 9 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_006717202.3 | 87.1% | 86.9% | (many diffs) |
| 10 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_006717203.4 | 87.1% | 86.9% | (many diffs) |
| 11 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NM_001145369.1 | 85.6% | 85.5% | 0_1ins393;2343delT |
| 12 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_006717204.3 | 85.6% | 85.5% | 0_1ins393;2343delT |
| 13 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_017014956.1 | 83% | 81.7% | (many diffs) |
| 14 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NM_001145370.1 | 80.6% | 80.6% | 0_1ins393;608_609ins135;2208delT |
| 15 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | XM_017014957.1 | 80.6% | 80.6% | 0_1ins393;608_609ins135;2208delT |
| 16 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NM_001145371.2 | 68.5% | 68.4% | 0_1ins861;1875delT |
| 17 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NM_001145372.2 | 63.5% | 63.5% | 0_1ins861;140_141ins135;1740delT |
| 18 | human | 5774 | PTPN3 | protein tyrosine phosphatas... | NR_026918.1 | 23.5% | (many diffs) | |
| 19 | mouse | 545622 | Ptpn3 | protein tyrosine phosphatas... | NM_011207.2 | 87.1% | 91.8% | (many diffs) |
| 20 | mouse | 545622 | Ptpn3 | protein tyrosine phosphatas... | XM_006538098.2 | 87.1% | 91.8% | (many diffs) |
| 21 | mouse | 545622 | Ptpn3 | protein tyrosine phosphatas... | XM_006538099.3 | 87.1% | 91.8% | (many diffs) |
| 22 | mouse | 545622 | Ptpn3 | protein tyrosine phosphatas... | XM_006538100.2 | 87.1% | 91.8% | (many diffs) |
| 23 | mouse | 545622 | Ptpn3 | protein tyrosine phosphatas... | XM_006538097.3 | 85% | 89.7% | (many diffs) |
| 24 | mouse | 545622 | Ptpn3 | protein tyrosine phosphatas... | XM_006538101.3 | 74.8% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2808
- ORF length:
- 2739
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacctcccgg ttacgtgcgt tgggtggaag aattaataat atacgcacct 121 cggagttacc caaagagaaa actcgatcag aagtcatttg cagcatccac tttttagatg 181 gcgtggtaca gacctttaaa gttactaaac aagacactgg ccaggttctt ctggatatgg 241 tgcacaacca cctgggtgtg actgaaaagg aatattttgg tttacagcat gatgacgact 301 ccgtggactc tcctagatgg ctggaagcaa gcaaagccat caggaagcag ttaaaaggag 361 gtttcccctg taccctgcat tttcgagtaa gattttttat acctgatccc aacacactgc 421 agcaagaaca aaccaggcac ttgtatttct tacaactgaa gatggatatt tgcgaaggaa 481 ggttaacctg ccctcttaac tcagcagtgg ttctagcgtc ctatgccgta caatctcatt 541 ttggagacta taattcttcc atacatcatc caggctatct ttccgatagt cactttatac 601 ccgatcaaaa tgaggacttt ttaacaaaag tcgaatctct gcatgagcag cacagtgggc 661 taaaacaatc agaagcagaa tcctgctata tcaacatagc gcggaccctc gacttctatg 721 gagtagaact gcacagtggt agggatctgc acaatttaga cctaatgatt ggaattgctt 781 ccgcgggtgt tgctgtgtac cgaaaataca tttgcacaag tttctatcct tgggtgaaca 841 ttctcaaaat ttctttcaaa aggaaaaagt tcttcataca tcagcgacag aaacaggctg 901 aatccaggga acatattgtg gccttcaaca tgctgaatta ccgatcttgc aaaaacttgt 961 ggaaatcctg tgttgagcac catacgttct ttcaggcaaa gaagctacta cctcaggaaa 1021 agaatgttct gtctcagtac tggactatgg gctctcggaa caccaaaaag tcggtaaata 1081 accaatattg caaaaaggtg attggcggga tggtgtggaa cccagccatg cggagatcct 1141 tatcagtgga gcacttagaa accaagagtc tgccttctcg ttcccctccc attactccca 1201 actggcgaag tcctcggctc cggcacgaaa tccgaaagcc acgccactct tctgcagata 1261 accttgcaaa tgaaatgacc tacatcacgg aaacggaaga tgtattttac acgtacaagg 1321 gctctctggc ccctcaagac agcgattctg aagtttctca gaaccgaagc ccgcaccaag 1381 agagtttatc cgagaacaat ccggcacaaa gctacctgac ccagaagtca tccagttctg 1441 tgtctccatc ttcaaatgct ccaggctcct gctcacctga cggcgttgat cagcagctct 1501 tagatgactt ccacagggtg accaaagggg gctccaccga ggacgccagc cagtactact 1561 gtgacaagaa tgataatggt gacagctact tagtcttgat ccgtatcaca ccagatgaag 1621 atggaaaatt tggatttaat cttaagggag gagtggatca aaagatgcct cttgtggtat 1681 caaggataaa cccagagtca cctgcggaca cctgcattcc taagctgaac gaaggggatc 1741 aaatcgtgtt aatcaatggc cgggacatct cagaacacac gcatgaccaa gtggtgatgt 1801 tcatcaaagc cagccgggag tcccactcac gggagctggc cctggtgatc aggaggagag 1861 ctgtccgctc atttgctgac ttcaagtctg aagatgaact gaaccagctt ttccccgaag 1921 ccattttccc catgtgtccg gagggtgggg acactttgga gggatccatg gcacagctaa 1981 agaagggcct cgaaagcggg acggtgctga tccagtttga gcaactctac agaaaaaagc 2041 caggtttggc catcacgttt gcaaagctgc ctcaaaattt ggacaaaaac cgatataaag 2101 atgtgctgcc ttatgacacc acccgggtat tattgcaggg aaatgaagat tatattaatg 2161 caagttacgt gaacatggaa attcctgctg ctaaccttgt gaacaagtac atcgccactc 2221 aggggcccct gccgcatacc tgtgcacagt tttggcaggt tgtctgggat cagaagttgt 2281 cactcattgt catgttgacg actctcacag aacgagggcg gaccaaatgt caccagtact 2341 ggccagatcc ccccgacgtc atgaaccacg gcggctttca catccagtgt cagtcagagg 2401 actgcaccat cgcctatgtg tcccgagaaa tgctggtcac aaacacccag accggggaag 2461 aacacacagt gacacatctc cagtacgtcg catggcctga ccacggtgtg cccgatgact 2521 cctccgactt tctggaattt gtaaactatg tgaggtctct gagagtggac agcgagcccg 2581 tcctagttca ctgcagtgct ggaataggtc gaaccggtgt gttggtcact atggaaacag 2641 ccatgtgcct aactgagagg aacctgccca tttacccact ggatattgtc cgaaaaatgc 2701 gagaccagcg cgccatgatg gtgcagacat caagccagta caagtttgtg tgtgaagcga 2761 ttcttcgtgt gtatgaagaa ggtttagtcc aaatgctgga tccagtttgc caactttctt 2821 gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta 2881 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta