Construct: ORF ccsbBroad304_13948

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006970.1_s304c1, BRDN0000402703
Derived from:
ccsbBroadEn_13948
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS12 (6206)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13948
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6206 RPS12 ribosomal protein S12 NM_001016.4 99.4% 99.2% 394A>G;396A>G
2 mouse 20042 Rps12 ribosomal protein S12 NM_011295.6 90.9% 99.2% (many diffs)
3 mouse 237433 Gm4925 predicted gene 4925 NM_001037166.2 86.4% 88.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
462
ORF length:
396
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cgaggaaggc attgctgctg gaggtgtaat ggacgttaat actgctttac
121aagaggttct gaagactgcc ctcatccacg atggcctagc acgtggaatt cgcgaagctg
181ccaaagcctt agacaagcgc caagcccatc tttgtgtgct tgcatccaac tgtgatgagc
241ctatgtatgt caagttggtg gaggcccttt gtgctgaaca ccaaatcaac ctaattaagg
301ttgatgacaa caagaaacta ggagaatggg taggcctttg taaaattgac agagagggga
361aaccccgtaa agtggttggt tgcagttgtg tagtagttaa ggactatggc aaggagtctc
421aggccaagga tgtcattgaa gagtatttca aatgcaagga gtgcccaact ttcttgtaca
481aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
541ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)