Construct: ORF ccsbBroad304_13970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004106.1_s304c1, BRDN0000462262
- Derived from:
- ccsbBroadEn_13970
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TMF1 (7110)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13970
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7110 | TMF1 | TATA element modulatory fac... | NM_007114.3 | 99.9% | 54.2% | 1242C>T;1777_1778insA |
| 2 | human | 7110 | TMF1 | TATA element modulatory fac... | NM_001363879.1 | 99.6% | 54.1% | 1242C>T;1348_1356delGTAACTCTA;1786_1787insA |
| 3 | mouse | 232286 | Tmf1 | TATA element modulatory fac... | NM_001081111.2 | 85.5% | 45.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttggttc aacgcctccc agctctccag cttcgctaag caggccctgt 121 cccaggccca gaagtctatt gacagggttc tggacatcca ggaagaggag ccgagcatct 181 gggccgagac cattccgtat ggagagccgg gaataagttc ccctgtcagt ggaggatggg 241 atacttcaac ctgggggttg aaatcaaaca ctgaacctca gagtccacca atagcctctc 301 ctaaagcaat cacaaagcca gttcggagga ctgtggtcga tgaatctgaa aatttcttca 361 gtgcctttct ctcgccaact gatgtccaga ccattcagaa gagtccagtg gtatcaaaac 421 ctccagcaaa atcacaacga ccagaagaag aagtgaaaag cagcttacat gaatccttgc 481 acattggcca gtcaagaact cctgaaacaa ctgaatcaca agtaaaagac tcttctttgt 541 gtgtttcagg ggaaactctg gcagcaggta cttcatcacc taaaactgaa ggcaagcacg 601 aagaaactgt taataaagaa tcggatatga aggtgccaac tgtaagtttg aaagtatctg 661 aaagtgtaat tgatgtgaaa acaactatgg aaagtatatc taatacgtct acgcagtctc 721 tcacagcaga aacaaaggac atagctttgg aacctaagga acaaaaacat gaagacaggc 781 agagcaatac accttctcct cctgttagta ccttttcatc aggtacttct accaccagtg 841 atattgaagt tttagatcat gaaagtgtaa taagtgagag ctcagcgagc tcgagacaag 901 agactacaga ttcaaaatca agtcttcact tgatgcagac atcttttcag cttctctctg 961 catctgcttg tcctgaatat aatcgtttag atgatttcca aaaactcact gagagttgct 1021 gttcatctga tgcttttgaa agaatagact catttagtgt acagtcatta gatagccgga 1081 gtgtaagtga aatcaattca gatgatgaat tgtcaggcaa gggatatgct ttagtgccta 1141 ttatagttaa ttcttcaact ccaaagtcta aaacagttga atctgctgaa ggaaaatctg 1201 aagaagtaaa tgaaacatta gttataccca ctgaggaagc agaaatggaa gaaagtggac 1261 gaagtgcaac tcctgttaac tgtgaacagc ctgatatctt ggtttcttct acaccaataa 1321 atgaaggaca gactgtgtta gacaaggtgg ctgagcagtg tgaacctgct gaaagtcagc 1381 cagaagcact ttctgagaag gaagatgttt gcaagacagt tgaatttctg aatgaaaagc 1441 tggaaaaaag ggaggctcag ttattatctc ttagtaagga aaaagcactt ctagaagaag 1501 cttttgataa cctgaaagat gaaatgttca gagtgaaaga agaaagcagt agcatttctt 1561 ccttgaaaga tgagtttact caaagaattg cagaagcaga aaagaaagtt caactagcct 1621 gcaaagagag agatgctgct aaaaaggaaa tcaaaaacat aaaagaagaa cttgccacta 1681 gattaaatag tagtgaaact gcagaccttt tgaaagagaa agatgagcag atccgagggt 1741 taatggaaga aggagaaaaa ctttcaaaac agcagctgca caattctaac atcatcaaga 1801 aattaagagc taaagacaag gagaatgaaa atatggttgc aaagcatgaa caaaaaagtt 1861 aaagagctag aagaggagtt gcagcatttg aaacaggtcc ttgatggcaa agaagaggtt 1921 gagaaacaac atagagaaaa tattaaaaaa ctaaattcca tggtagaacg ccaagagaaa 1981 gatcttggcc gtcttcaggt agacatggat gaacttgaag aaaagaaccg aagtattcag 2041 gctgccctgg atagtgcata caaagaactt actgatcttc acaaagccaa tgctgcaaag 2101 gatagtgagg cacaggaagc tgctctgagc cgtgaaatga aagctaaaga agaactttct 2161 gcagcattag agaaggccca agaagaagcc cgtcagcagc aagaaacatt agccattcaa 2221 gtgggggacc ttaggcttgc attgcagcgt acagaacaag cggctgccag aaaggaggat 2281 tatttacgcc atgagatcgg tgaacttcag cagagactcc aggaagcaga gaatcgaaac 2341 caggaactga gtcaaagtgt ttcatcaaca acaagaccat tgcttcgaca aatagaaaat 2401 ttgcaagcaa ccctgggatc ccagacatcg tcgtgggaga aattagagaa gaatctttct 2461 gataggcttg gtgaatccca gaccttgctg gcagcagcag ttgagagaga acgtgcagct 2521 acagaagaac tccttgctaa caaaattcag atgtcttcca tggagtcaca gaattctctt 2581 ttaagacagg aaaacagtag atttcaagcc cagctagaat cagagaaaaa taggctgtgt 2641 aaactggagg atgagaacaa taggtaccag gttgaattgg aaaacctaaa agatgaatat 2701 gtaagaacac ttgaagagac gaggaaagaa aagacattgt tgaatagtca gttagaaatg 2761 gaaagaatga aagttgaaca agaaaggaag aaagccattt ttactcaaga aacaataaaa 2821 gaaaaggaac gcaagccatt ttctgtttct agcactccca ccatgtcacg ctcaagttca 2881 ataagtggtg ttgatatggc aggactacag acatcttttc tgtctcagga tgagtctcat 2941 gatcactcat ttggaccaat gcctatatca gcaaatggaa gcaatcttta tgatgctgta 3001 aggatgggag caggatcaag cataattgaa aacctacagt ctcagctaaa gctaagggaa 3061 ggggaaatca ctcatttaca gctagaaatt ggcaatctag aaaaaactcg atcaataatg 3121 gctgaagaac tagttaaatt aacaaatcaa aatgatgaac ttgaagagaa ggtgaaggag 3181 atacccaaac ttagaactca gctaagagat ttggatcaaa ggtacaacac tattctgcag 3241 atgtatggag aaaaagcaga agaggcagaa gaacttcgat tagatctcga agatgtaaaa 3301 aatatgtaca aaactcaaat agatgaactt ttaagacaaa gtctcagttt gccaactttc 3361 ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag 3421 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta