Construct: ORF ccsbBroad304_13983

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008558.1_s304c1, BRDN0000399720
Derived from:
ccsbBroadEn_13983
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
YWHAE (7531)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13983
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7531 YWHAE tyrosine 3-monooxygenase/tr... NM_006761.5 99.7% 1.5% 10delC;764A>C
2 human 7531 YWHAE tyrosine 3-monooxygenase/tr... XM_005256784.4 93% 1.6% 10delC;716A>G;718_720delTCCins48
3 human 7531 YWHAE tyrosine 3-monooxygenase/tr... XM_017025005.2 84.7% 4% 0_1ins65;650A>G;652_654delTCCins48
4 human 7531 YWHAE tyrosine 3-monooxygenase/tr... NR_024058.1 41% (many diffs)
5 human 649395 YWHAEP1 tyrosine 3-monooxygenase/tr... NR_029404.1 36.9% (many diffs)
6 mouse 22627 Ywhae tyrosine 3-monooxygenase/tr... NM_009536.4 93.2% 1.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
144
ORF length:
78
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tgatgagagg atctggtgta ccaggcgaag ctggccgagc aggctgagcg
121atacgacgaa atggtggagt caatgaagaa agtagcaggg atggatgtgg agctgacagt
181tgaagaaaga aacctcctat ctgttgcata taagaatgtg attggagcta gaagagcctc
241ctggagaata atcagcagca ttgaacagaa agaagaaaac aagggaggag aagacaagct
301aaaaatgatt cgggaatatc ggcaaatggt tgagactgag ctaaagttaa tctgttgtga
361cattctggat gtactggaca aacacctcat tccagcagct aacactggcg agtccaaggt
421tttctattat aaaatgaaag gggactacca caggtatctg gcagaatttg ccacaggaaa
481cgacaggaag gaggctgcgg agaacagcct agtggcttat aaagctgcta gtgatattgc
541aatgacagaa cttccaccaa cgcatcctat tcgcttaggt cttgctctca atttttccgt
601attctactac gaaattctta attcccctga ccgtgcctgc aggttggcaa aagcagcttt
661tgatgatgca attgcagaac tggatacgct gagtgaagaa agctataagg actctacact
721tatcatgcag ttgttacgtg ataatctgac actatggact tcagacatgc agggtgacgg
781tgaagagcag aataaagaag cgctgcagga cgtggaagac gaaaatccgt gcccaacttt
841cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta
901gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)