Transcript: Mouse NM_009536.4

Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Ywhae (22627)
Length:
2078
CDS:
142..909

Additional Resources:

NCBI RefSeq record:
NM_009536.4
NBCI Gene record:
Ywhae (22627)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009536.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426609 GATTCGGGAGTACCGGCAAAT pLKO_005 384 CDS 100% 10.800 15.120 N Ywhae n/a
2 TRCN0000427753 TAGGTTCTTTACGACACTAAC pLKO_005 1173 3UTR 100% 10.800 15.120 N Ywhae n/a
3 TRCN0000012384 CGACGAAATGGTGGAATCAAT pLKO.1 201 CDS 100% 5.625 7.875 N Ywhae n/a
4 TRCN0000430607 AGAATCAGTGAGACGAAATAA pLKO_005 899 CDS 100% 15.000 12.000 N Ywhae n/a
5 TRCN0000062233 CGCTGAGTGAAGAAAGCTATA pLKO.1 764 CDS 100% 10.800 8.640 N YWHAE n/a
6 TRCN0000290141 CGCTGAGTGAAGAAAGCTATA pLKO_005 764 CDS 100% 10.800 8.640 N YWHAE n/a
7 TRCN0000012383 CCAGACATATTTGACAGTATT pLKO.1 1862 3UTR 100% 13.200 9.240 N Ywhae n/a
8 TRCN0000012387 GCTGAGTGAAGAAAGCTATAA pLKO.1 765 CDS 100% 13.200 9.240 N Ywhae n/a
9 TRCN0000425791 ATGCAGCTGCTACGTGATAAC pLKO_005 802 CDS 100% 10.800 7.560 N Ywhae n/a
10 TRCN0000012385 CAGTTGAAGAACGAAACCTTT pLKO.1 254 CDS 100% 4.950 3.465 N Ywhae n/a
11 TRCN0000012386 GATGGTGAAGAGCAGAATAAA pLKO.1 853 CDS 100% 15.000 9.000 N Ywhae n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009536.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13983 pDONR223 100% 93.2% 1.5% None (many diffs) n/a
2 ccsbBroad304_13983 pLX_304 0% 93.2% 1.5% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000472772 CCTCGGTCAGCGATTTATAACCTT pLX_317 73.7% 93.2% 1.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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