Construct: ORF ccsbBroad304_13989

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013505.1_s304c1, BRDN0000461201
Derived from:
ccsbBroadEn_13989
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PABPN1 (8106)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13989
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8106 PABPN1 poly(A) binding protein nuc... NM_004643.3 99.3% 99% 3_8delGGCGGC
2 human 8106 PABPN1 poly(A) binding protein nuc... NM_001360551.2 95.3% 95% 3_8delGGCGGC;883_885delTCA;888_888delAins34
3 human 100529063 BCL2L2-PABPN1 BCL2L2-PABPN1 readthrough NM_001199864.2 73.4% 50.3% (many diffs)
4 human 8106 PABPN1 poly(A) binding protein nuc... NM_001360552.1 58.5% 58.5% 0_1ins378
5 mouse 54196 Pabpn1 poly(A) binding protein, nu... NM_019402.2 92.7% 95.7% (many diffs)
6 mouse 54196 Pabpn1 poly(A) binding protein, nu... XM_006519272.3 55% 57.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
978
ORF length:
912
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggcggcggcg gcagcagcag cgggggctgc gggcggtcgg ggctccgggc
121cggggcggcg gcgccatctt gtgcccgggg ccggtgggga ggccggggag ggggccccgg
181ggggcgcagg ggactacggg aacggcctgg agtctgagga actggagcct gaggagctgc
241tgctggagcc cgagccggag cccgagcccg aagaggagcc gccccggccc cgcgcccccc
301cgggagctcc gggccctggg cctggttcgg gagcccccgg cagccaagag gaggaggagg
361agccgggact ggtcgagggt gacccggggg acggcgccat tgaggacccg gagctggaag
421ctatcaaagc tcgagtcagg gagatggagg aagaagctga gaagctaaag gagctacaga
481acgaggtaga gaagcagatg aatatgagtc cacctccagg caatgctggc ccggtgatca
541tgtccattga ggagaagatg gaggctgatg cccgttccat ctatgttggc aatgtggact
601atggtgcaac agcagaagag ctggaagctc actttcatgg ctgtggttca gtcaaccgtg
661ttaccatact gtgtgacaaa tttagtggcc atcccaaagg gtttgcgtat atagagttct
721cagacaaaga gtcagtgagg acttccttgg ccttagatga gtccctattt agaggaaggc
781aaatcaaggt gatcccaaaa cgaaccaaca gaccaggcat cagcacaaca gaccggggtt
841ttccacgagc ccgctaccgc gcccggacca ccaactacaa cagctcccgc tctcgattct
901acagtggttt taacagcagg ccccggggtc gcgtctacag gggccgggct agagcgacat
961catggtattc cccttactac ccaactttct tgtacaaagt ggttggtaag cctatcccta
1021accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1081agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)