Construct: ORF ccsbBroad304_13993

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004735.1_s304c1, BRDN0000410749
Derived from:
ccsbBroadEn_13993
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SUPT3H (8464)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13993
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_181356.3 99.8% 98.7% 969delG
2 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_011514949.3 97.1% 96% (many diffs)
3 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_017011369.2 97.1% 96% (many diffs)
4 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_017011370.1 97.1% 96% (many diffs)
5 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_001350329.2 90.1% 79.4% (many diffs)
6 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_003599.4 90.1% 79.4% (many diffs)
7 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_017011371.1 90.1% 79.4% (many diffs)
8 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_024446572.1 90.1% 79.4% (many diffs)
9 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_001350326.2 90.1% 89% 35_36ins96;873delG
10 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_001350325.2 89.5% 78.9% (many diffs)
11 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_001350324.2 87.6% 77% (many diffs)
12 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_011514951.3 87.6% 77% (many diffs)
13 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_011514952.2 87.6% 77% (many diffs)
14 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_011514953.3 87.6% 77% (many diffs)
15 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_011514954.3 87.6% 77% (many diffs)
16 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_926320.1 78.5% 1_51del;885_1025del;1115_1116ins60
17 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_001350327.2 70.3% 69.2% 0_1ins291;678delG
18 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_926321.1 68.9% (many diffs)
19 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XM_017011374.2 67.9% 66.6% (many diffs)
20 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_926319.3 67.8% 1_51del;1020delG;1036_1449del
21 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NR_146632.1 59.5% (many diffs)
22 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NR_146634.1 56.2% (many diffs)
23 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NR_146633.1 55.2% (many diffs)
24 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NR_146635.1 54.6% (many diffs)
25 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_001743692.1 53.4% (many diffs)
26 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... NM_001261823.2 50.2% 49% 0_1ins489;480delG
27 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_002956310.1 49.6% (many diffs)
28 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_001743688.1 41.1% (many diffs)
29 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_001743691.1 33.8% (many diffs)
30 human 8464 SUPT3H SPT3 homolog, SAGA and STAG... XR_001743685.1 32.8% 1_51del;1020delG;1036_2992del
31 mouse 109115 Supt3 suppressor of Ty 3 NM_178652.2 68.8% 63.6% (many diffs)
32 mouse 109115 Supt3 suppressor of Ty 3 XM_006523480.2 67.3% 52.6% (many diffs)
33 mouse 109115 Supt3 suppressor of Ty 3 XM_006523479.3 66% 64.5% (many diffs)
34 mouse 109115 Supt3 suppressor of Ty 3 XM_006523476.3 65.1% 55.4% (many diffs)
35 mouse 109115 Supt3 suppressor of Ty 3 XM_006523475.3 64.7% 53.4% (many diffs)
36 mouse 109115 Supt3 suppressor of Ty 3 XM_006523477.2 62.2% 56.4% (many diffs)
37 mouse 109115 Supt3 suppressor of Ty 3 XM_006523478.3 62.2% 56.4% (many diffs)
38 mouse 109115 Supt3 suppressor of Ty 3 XM_006523474.3 60.1% 57.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
65
ORF end:
1040
ORF length:
975
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttgcatggtg gtctttggaa caacgttctt caactttgac agcttcatag ggaaaacaga
121ttctatagaa ataattggaa aatcagtttt tccctattgt tatcacaata tccttccact
181gctgaatgat tctggcaggt attctttagg tgatgctaga aggcctcttc atgaaacagc
241agttttggta gaagatgtgg tacacactca gttaattaat ctgttacagc aagctgctga
301agtttctcag ctgcggggag caagggtaat cactcctgaa gatcttctgt ttttgatgcg
361caaagataag aaaaaactta gaagactgct aaaatacatg tttatccgag actacaaatc
421aaagattgtc aaaggcatcg atgaggatga tcttctcgaa gacaaattga gtggcagcaa
481taatgcgaac aaaagacaaa agattgctca ggacttcctc aactctattg accagacagg
541agaactttta gcaatgtttg aagatgacga aattgatgaa gttaaacaag aaagaatgga
601gagagcagaa agacaaactc gaattatgga ttcagctcaa tatgcagaat tctgtgaaag
661tcgacaatta agtttctcca aaaaagcttc caaatttcga gactggttgg actgcagcag
721tatggagata aaacccaatg ttgtcgcaat ggaaatctta gcatatttag cgtatgaaac
781tgtggcacag ttagtggatc tggctcttct tgtgaggcaa gacatggtaa ccaaggcagg
841ggaccccttc agccatgcca tttctgcaac cttcattcag tatcacaact ctgctgagag
901cactgcagcc tgtggtgttg aggctcacag cgatgccatc cagccctgcc acatcagaga
961ggccattcga cgctacagcc acaggattgg cccactttcc ccattcacaa atgcctaccg
1021caggaatggg atgcttttct agcctgctgc ccaactttct tgtacaaagt ggttggtaag
1081cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg
1141tggcgcgtta agtcgacaat caacctctgg atta

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