Construct: ORF ccsbBroad304_14004
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008737.1_s304c1, BRDN0000398940
- Derived from:
- ccsbBroadEn_14004
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCNK (8812)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14004
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8812 | CCNK | cyclin K | NM_001099402.2 | 60.9% | 52.4% | 918_1590del;1724_1725delGGinsA;1736_1740delTGAGA |
| 2 | human | 8812 | CCNK | cyclin K | XM_005268154.4 | 60.9% | 52.4% | 918_1590del;1724_1725delGGinsA;1736_1740delTGAGA |
| 3 | human | 8812 | CCNK | cyclin K | XM_011537275.2 | 60.9% | 52.4% | 918_1590del;1724_1725delGGinsA;1736_1740delTGAGA |
| 4 | mouse | 12454 | Ccnk | cyclin K | NM_009832.2 | 55.3% | 53.1% | (many diffs) |
| 5 | mouse | 12454 | Ccnk | cyclin K | XM_006515443.3 | 55.3% | 53.1% | (many diffs) |
| 6 | mouse | 12454 | Ccnk | cyclin K | XM_006515444.3 | 55.3% | 53.1% | (many diffs) |
| 7 | mouse | 12454 | Ccnk | cyclin K | XM_006515445.2 | 55.3% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagaataaa gaaaattcaa gcccttcagt aacttcagca aacctggacc 121 acacaaagcc atgttggtac tgggataaga aagacttggc tcatacaccc tcacaacttg 181 aaggacttga tccagccacc gaggcccggt accgccgaga gggcgctcgg ttcatctttg 241 atgtgggcac acgtttgggg ctacactatg ataccctggc aactggaata atttattttc 301 atcgcttcta tatgtttcat tccttcaagc aattcccaag atatgtgaca ggagcctgtt 361 gcctctttct ggctgggaaa gtagaagaaa caccaaaaaa atgtaaagat atcatcaaaa 421 cagctcgtag tttattaaat gatgtacaat ttggccagtt tggagatgac ccaaaggagg 481 aagtaatggt tctggagaga atcttactgc agaccatcaa gtttgattta caggtagaac 541 atccatacca gttcctacta aaatatgcaa agcaactcaa aggtgataaa aacaaaattc 601 aaaagttggt tcaaatggca tggacatttg taaatgacag tctctgcacc accttgtcac 661 tgcagtggga accagagatc atagcagtag cagtgatgta tctcgcagga cgtttgtgca 721 aatttgaaat acaagaatgg acctccaaac ccatgtatag gagatggtgg gagcagtttg 781 ttcaagatgt cccggtcgac gttttggaag acatctgcca ccaaatcctg gatctttact 841 cacaaggaaa acaacagatg cctcatcaca ccccccatca gctgcaacag cccccatctc 901 ttcagcctac accacaagtg ccgcaagtac agcagtcaca gccgtctcaa agctccgaac 961 catcccagcc ccagcagaag gaccccctca tcctcctcca gggttgggcc tgccgccagc 1021 cagctaccca cctcctgccg tcccccctgg aggacagcct cctgtgcccc cgcccattcc 1081 cccacccggc atgcctccag ttggggggct ggggcagcag cctggatgcc caactttctt 1141 gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta 1201 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta