Transcript: Human XM_005268154.4

PREDICTED: Homo sapiens cyclin K (CCNK), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CCNK (8812)
Length:
2523
CDS:
256..1998

Additional Resources:

NCBI RefSeq record:
XM_005268154.4
NBCI Gene record:
CCNK (8812)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005268154.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045185 CGCAGGACGTTTGTGCAAATT pLKO.1 894 CDS 100% 13.200 18.480 N CCNK n/a
2 TRCN0000288770 CGCAGGACGTTTGTGCAAATT pLKO_005 894 CDS 100% 13.200 18.480 N CCNK n/a
3 TRCN0000045183 GCAGACCATCAAGTTTGATTT pLKO.1 699 CDS 100% 13.200 10.560 N CCNK n/a
4 TRCN0000288769 GCAGACCATCAAGTTTGATTT pLKO_005 699 CDS 100% 13.200 10.560 N CCNK n/a
5 TRCN0000045187 CCACCAAATCCTGGATCTTTA pLKO.1 1008 CDS 100% 13.200 9.240 N CCNK n/a
6 TRCN0000288839 CCACCAAATCCTGGATCTTTA pLKO_005 1008 CDS 100% 13.200 9.240 N CCNK n/a
7 TRCN0000045184 GCCATGTTGGTACTGGGATAA pLKO.1 318 CDS 100% 10.800 7.560 N CCNK n/a
8 TRCN0000288772 GCCATGTTGGTACTGGGATAA pLKO_005 318 CDS 100% 10.800 7.560 N CCNK n/a
9 TRCN0000045186 GCAAAGCAACTCAAAGGTGAT pLKO.1 757 CDS 100% 4.050 2.835 N CCNK n/a
10 TRCN0000288841 GCAAAGCAACTCAAAGGTGAT pLKO_005 757 CDS 100% 4.050 2.835 N CCNK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005268154.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14004 pDONR223 100% 60.9% 52.4% None 918_1590del;1724_1725delGGinsA;1736_1740delTGAGA n/a
2 ccsbBroad304_14004 pLX_304 0% 60.9% 52.4% V5 (not translated due to frame shift) 918_1590del;1724_1725delGGinsA;1736_1740delTGAGA n/a
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