Construct: ORF ccsbBroad304_14042

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008516.1_s304c1, BRDN0000460755
Derived from:
ccsbBroadEn_14042
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CWC27 (10283)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14042
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10283 CWC27 CWC27 spliceosome associate... NM_001297644.1 74.3% 2.5% 1_300del;306delA
2 human 10283 CWC27 CWC27 spliceosome associate... XM_011543095.3 65.3% 2.7% (many diffs)
3 human 10283 CWC27 CWC27 spliceosome associate... NM_001364478.1 64.4% 1.9% (many diffs)
4 human 10283 CWC27 CWC27 spliceosome associate... NM_005869.4 61.5% 2% (many diffs)
5 human 10283 CWC27 CWC27 spliceosome associate... NM_001297645.1 60.3% 1.5% (many diffs)
6 human 10283 CWC27 CWC27 spliceosome associate... XM_011543096.2 60% 2.8% (many diffs)
7 human 10283 CWC27 CWC27 spliceosome associate... NM_001318000.1 55.5% 2.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
204
ORF length:
138
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc aatgctggtt ctcatgataa tggcagccag tttttcttca cactgggtcg
121agcagatgaa cttaacaata agcataccat ctttggaaag gttacagggg atacagtata
181taacatgttg cgactgtcag aagtagacat tgatgatgac gaaagaccac ataatccaca
241caaaataaaa agctgtgagg ttttgtttaa tccttttgat gacatcattc caagggaaat
301taaaaggctg aaaaaagaga aaccagagga ggaagtaaag aaattgaaac ccaaaggcac
361aaaaaatttt agtttacttt catttggaga ggaagctgag gaagaagagg aggaagtaaa
421tcgagttagt cagagcatga agggcaaaag caaaagtagt catgacttgc ttaaggatga
481tccacatctc agttctgttc cagttgtaga aagtgaaaaa ggtgatgcac cagatttagt
541tgatgatgga gaagatgaaa gtgcagagca tgatgaatat attgatggtg atgaaaagaa
601cctgatgaga gaaagaattg ccaaaaaatt aaaaaaggac acaagtgcga atgttaaatc
661agctggagaa ggagaagtgg agaagaaatc agtcagccgc agtgaagagc tcagaaaaga
721agcaagacaa ttaaaacggg aactcttagc agcaaaacaa aaaaaagtag aaaatgcagc
781aaaacaagca gaaaaaagaa gtgaagagga agaagcccct ccagatggtg ctgttgccga
841atacagaaga gaaaagcaaa agtatgaagc tttgaggaag caacagtcaa agaagggaac
901ttcccgggaa gatcaggatg tcacatgtac ttcagtttgc ccaactttct tgtacaaagt
961ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag
1021cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)