Transcript: Human NM_005869.4

Homo sapiens CWC27 spliceosome associated cyclophilin (CWC27), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
CWC27 (10283)
Length:
2065
CDS:
218..1636

Additional Resources:

NCBI RefSeq record:
NM_005869.4
NBCI Gene record:
CWC27 (10283)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005869.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000350972 GCTGGAAATGTGCCTACAATG pLKO_005 1663 3UTR 100% 10.800 8.640 N CWC27 n/a
2 TRCN0000000136 CGAGCAGATGAACTTAACAAT pLKO.1 572 CDS 100% 5.625 3.938 N CWC27 n/a
3 TRCN0000338374 CGAGCAGATGAACTTAACAAT pLKO_005 572 CDS 100% 5.625 3.938 N CWC27 n/a
4 TRCN0000000138 GAACCTGATGAGAGAAAGAAT pLKO.1 1051 CDS 100% 5.625 3.938 N CWC27 n/a
5 TRCN0000338375 GAACCTGATGAGAGAAAGAAT pLKO_005 1051 CDS 100% 5.625 3.938 N CWC27 n/a
6 TRCN0000000139 ACGGGAACTCTTAGCAGCAAA pLKO.1 1189 CDS 100% 4.950 3.465 N CWC27 n/a
7 TRCN0000338376 ACGGGAACTCTTAGCAGCAAA pLKO_005 1189 CDS 100% 4.950 3.465 N CWC27 n/a
8 TRCN0000000135 TAATGATAACCAGAACTTGCT pLKO.1 1645 3UTR 100% 2.640 1.848 N CWC27 n/a
9 TRCN0000000137 AGACCACATAATCCACACAAA pLKO.1 677 CDS 100% 4.950 2.970 N CWC27 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005869.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14042 pDONR223 100% 61.5% 2% None (many diffs) n/a
2 ccsbBroad304_14042 pLX_304 0% 61.5% 2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000475809 GGGATCCATAGTTAGTCTAAAATC pLX_317 38.7% 61.5% 2% V5 (not translated due to prior stop codon) (many diffs) n/a
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