Construct: ORF ccsbBroad304_14048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004450.1_s304c1, BRDN0000461058
- Derived from:
- ccsbBroadEn_14048
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4D (10507)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14048
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10507 | SEMA4D | semaphorin 4D | NM_001142287.2 | 16% | 14.9% | (many diffs) |
| 2 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371198.1 | 16% | 14.9% | (many diffs) |
| 3 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371199.1 | 16% | 14.9% | (many diffs) |
| 4 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371200.1 | 16% | 14.9% | (many diffs) |
| 5 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371201.1 | 16% | 14.9% | (many diffs) |
| 6 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746156.1 | 11.1% | (many diffs) | |
| 7 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746153.1 | 11.1% | (many diffs) | |
| 8 | human | 10507 | SEMA4D | semaphorin 4D | NR_163886.1 | 8.8% | (many diffs) | |
| 9 | human | 10507 | SEMA4D | semaphorin 4D | NR_163887.1 | 8.7% | (many diffs) | |
| 10 | human | 10507 | SEMA4D | semaphorin 4D | NR_163884.1 | 8.7% | (many diffs) | |
| 11 | human | 10507 | SEMA4D | semaphorin 4D | NR_163885.1 | 8.6% | (many diffs) | |
| 12 | human | 10507 | SEMA4D | semaphorin 4D | NR_163888.1 | 8.5% | (many diffs) | |
| 13 | human | 10507 | SEMA4D | semaphorin 4D | NR_163889.1 | 8.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 438
- ORF length:
- 369
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggactcag aggtggaaac ggtctgtgtc cccaggtcct gcagacattc 121 gctttgtctg ggagaagaat gggcgagctc tggagacctg tgtccctgtg cagacccatg 181 cactgcccga tggcagggcc catgcactca gctggctgca ggacgccatc agggaaagcg 241 ctgagtatcg ctgctctgtc ctctcctcag cagggaacaa gacttcgaag gtgcaggttg 301 ctgtgatgag acctgaagtg acccaccagg agaggtggac cagagagctc tctgcctgga 361 gggctgtggc tgggnagcac gaccggatga tgcagagctg gaggaaggcg tgggaaagct 421 gtagcaagga caccctgttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 481 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 541 agtcgacaat caacctctgg atta