Construct: ORF ccsbBroad304_14054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008193.1_s304c1, BRDN0000403718
- Derived from:
- ccsbBroadEn_14054
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAI1 (10743)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14054
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10743 | RAI1 | retinoic acid induced 1 | NM_030665.4 | 49.6% | 49.2% | (many diffs) |
| 2 | human | 10743 | RAI1 | retinoic acid induced 1 | XM_017024025.1 | 48.1% | 47.7% | (many diffs) |
| 3 | human | 10743 | RAI1 | retinoic acid induced 1 | XM_017024026.1 | 48.1% | 47.7% | (many diffs) |
| 4 | human | 10743 | RAI1 | retinoic acid induced 1 | XM_017024027.1 | 48.1% | 47.7% | (many diffs) |
| 5 | human | 10743 | RAI1 | retinoic acid induced 1 | XM_017024028.2 | 48.1% | 47.7% | (many diffs) |
| 6 | mouse | 19377 | Rai1 | retinoic acid induced 1 | NM_001037764.1 | 41.1% | 42% | (many diffs) |
| 7 | mouse | 19377 | Rai1 | retinoic acid induced 1 | NM_009021.2 | 41.1% | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2964
- ORF length:
- 2898
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gtcttttcga gaaaggtgtg gtttccatgg caaacaacag aactaccagc 121 agacctcgca ggaaacatca cgcctagaga attacaggca gccgagtcag gccgggctaa 181 gctgcgaccg gcagcggctg ctcgccaagg actattataa cccgcagcct tacccgagct 241 atgagggtgg cgctggcacg ccctctggca ctgcagccgc ggtggccgcc gacaagtacc 301 accgaggcag caaggccctg cccacacagc aaggcctgca ggggaggccg gctttccctg 361 gctacggcgt ccaggacagc agcccctacc caggccgcta tgctggtgag gagagccttc 421 aggcttgggg ggccccacag ccaccacccc cacagccgca gccactacct gcaggggtgg 481 ccaagtatga tgagaacttg atgaaaaaga cagcagtgcc ccccagcagg cagtatgcag 541 agcagggcgc ccaggtgccc tttcggactc actccctgca cgtccagcag ccaccgccgc 601 cccagcagcc cctggcatac cccaagctcc aaaggcagaa gctgcagaac gacattgcct 661 cccctctgcc cttcccccag ggtacccact ttcctcagca ttcccagtcc ttccccacct 721 cctccaccta ctcctcctct gtccagggtg gtgggcaggg ggcccactcc tataagagtt 781 gcacagcacc gactgcccag ccccatgaca ggccgctgac tgccagctcc agcctggccc 841 cggggcagcg ggtccagaat cttcatgcct accagtcggg ccgcctcagc tatgaccagc 901 agcagcagca gcagcagcag cagcagcagc agcagcaagc ccttcagagc cggcaccatg 961 cccaggaaac cctccattac caaaacctcg ccaagtatca gcactacggg cagcaaggcc 1021 agggctactg ccagccggac gcagccgtcc ggaccccaga gcagtactac cagaccttca 1081 gccccagctc cagccactca cccgcccgct ccgtgggccg ctcaccttcc tacagttcca 1141 caccgtcgcc gctgatgcca aacctggaga actttcccta cagccagcag ccgctcagca 1201 ccggggcctt ccccgcaggg atcactgacc acagccactt catgcccctg ctcaatccct 1261 ccccaacgga tgccaccagc tctgtggaca cccaggctgg caactgcaag ccccttcaga 1321 aggacaagct ccctgagaac ctgctgtcgg atctcagcct gcagagcctc acggcgctga 1381 cctcacaggt ggagaacatc tccaacaccg tccagcagct gctgctctcc aaggctgctg 1441 tgccgcagaa gaaaggtgtc aagaacctcg tgtccaggac cccagagcag cataaaagcc 1501 agcactgcag ccccgaaggg agcggctact cagccgagcc cgcaggcaca ccgctgtcag 1561 agccgccgag cagcacgcca cagtccacgc atgcggagcc gcaggaggcc gactacctga 1621 gcggctccga ggacccactg gagcgcagct tcctctactg caaccaggcc cgtggcagcc 1681 ctgccagggt caacagcaac tcgaaggcca agcccgagtc cgtgtccacc tgttctgtga 1741 cctctcctga cgacatgtcc accaaatctg acgactcctt ccagagccta cacggcagtc 1801 tgccgctcaa cagcttctcc aagttcgtgg cgggtgagcg ggactgtccg cggctgctgc 1861 tcagcgccct ggcacaggag gacctggcct ccgagatcct ggggctgcag gaagccatcg 1921 gtgagaaggc cgacaaagct tgggctgaag cacccagcct ggtcaaggac agcagcaagc 1981 cacccttctc gctggagaac cacagcgcct gcctggactc tgtggccaag agtgcgtggc 2041 cccggcctgg ggagccagag gccctgcccg actccttgca gctggacaag ggcggcaatg 2101 ccaaggactt cagcccaggg ctgtttgaag acccttccgt ggccttcgct acgcctgacc 2161 ccaaaaagac aactggtcct ctctcctttg gtaccaagcc cacccttggg gttcctgctc 2221 cagaccccac tacagcagct tttgactgtt tcccggacac aaccgctgcc agctcagcgg 2281 acagcgccaa cccctttgcc tggccagagg aaaacctggg ggatgcttgt cccaggtggg 2341 gattgcaccc tggcgagctt accaagggcc tggagcaggg tgggaaggcc tcagatggca 2401 tcagcaaagg ggacacccat gaggcttcgg cctgcctggg cttccaggag gaggaccccc 2461 ctggggagaa ggtggcctcg ttgcccgggg acttcaagca ggaggaggtg ggtggggtga 2521 aggaggaggc aggtgggctg ctgcagtgcc ccgaggtggc caaggctgac cggtggctgg 2581 aggacagccg gcactgctgt tccaccgccg acttcgggga cctcccactg ctgccaccca 2641 ccagcaggaa ggaggacctg gaagctgagg aggagtactc ctccctatgt gagctcctgg 2701 gcagccccga gcagaggcct ggcatgcagg acccgctgtc acccaaggcc ccactcatct 2761 gcaccaagga ggaggtggag gaggtgctgg actccaaggc cggctggggc tctccgtgcc 2821 acctctcagg ggagtccgtc atcctgctgg gccctacagt gggcaccgag tcaaaggtcc 2881 agagctggtt tgagtcctcg ttatatagtg tatatatatg tatacatata catatatata 2941 atatatatga agactgtaaa tgttacccaa ctttcttgta caaagtggtt ggtaagccta 3001 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3061 gcgttaagtc gacaatcaac ctctggatta