Construct: ORF ccsbBroad304_14071

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009415.1_s304c1, BRDN0000461658
Derived from:
ccsbBroadEn_14071
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EPN2 (22905)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14071
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22905 EPN2 epsin 2 NM_148921.3 99.8% .3% 4delA;1031T>C
2 human 22905 EPN2 epsin 2 NM_014964.4 91% .3% 4delA;596_766del;1202T>C
3 human 22905 EPN2 epsin 2 NM_001102664.1 60.9% .5% 0_1ins683;347T>C
4 mouse 13855 Epn2 epsin 2 NM_001252189.1 84.5% .3% (many diffs)
5 mouse 13855 Epn2 epsin 2 NM_010148.3 82.8% .3% (many diffs)
6 mouse 13855 Epn2 epsin 2 XM_017314253.1 82.8% .3% (many diffs)
7 mouse 13855 Epn2 epsin 2 NM_001252188.1 77.1% .3% (many diffs)
8 mouse 13855 Epn2 epsin 2 XM_006532171.3 77.1% .3% (many diffs)
9 mouse 13855 Epn2 epsin 2 XM_006532163.3 75.7% .3% (many diffs)
10 mouse 13855 Epn2 epsin 2 XM_006532165.3 75.7% .3% (many diffs)
11 mouse 13855 Epn2 epsin 2 XM_006532166.3 75.7% .3% (many diffs)
12 mouse 13855 Epn2 epsin 2 XM_006532167.3 75.7% .3% (many diffs)
13 mouse 13855 Epn2 epsin 2 XM_006532168.3 75.7% .3% (many diffs)
14 mouse 13855 Epn2 epsin 2 XM_006532169.3 75.7% .3% (many diffs)
15 mouse 13855 Epn2 epsin 2 XM_006532170.3 75.7% .3% (many diffs)
16 mouse 13855 Epn2 epsin 2 XM_011248711.2 75.7% .3% (many diffs)
17 mouse 13855 Epn2 epsin 2 XM_011248712.2 75.7% .3% (many diffs)
18 mouse 13855 Epn2 epsin 2 XM_011248713.2 75.7% .3% (many diffs)
19 mouse 13855 Epn2 epsin 2 XM_011248714.2 75.7% .3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
96
ORF length:
27
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcgacttcgt ctatcagacg gcagatgaaa aacatcgtga acaattactc
121agaggcagaa atcaaagtcc gggaagccac ctccaatgac ccgtggggcc cgtccagttc
181tctgatgacc gagattgccg acctgaccta caacgtggtg gccttctcgg agatcatgag
241catggtgtgg aagcggctga atgaccatgg caagaactgg cggcatgtgt acaaggcgct
301gaccctgctg gactacctca tcaagacagg ctccgaacgt gtggcccagc agtgccggga
361gaacatcttc gccatccaga ccctgaagga cttccagtac attgaccgag atggcaagga
421ccagggcatc aatgtgcgtg agaagtcaaa gcaactggtg gctctcctca aggacgagga
481acggttgaag gctgagaggg cccaggctct caaaaccaaa gagcgcatgg cccaggttgc
541cactggcatg ggcagcaacc agatcacctt tgggcgaggc tccagccagc ccaacctctc
601caccagccac tcggagcagg agtatggcaa ggccgggggc tccccggcct cctaccatgg
661ctccacctcc ccgcgagtgt cctccgagct ggagcaagcc cggccccaga ctagtggaga
721agaggagctt cagctgcagc tggcacttgc catgagcaga gaagtggctg agcaggaaga
781acgcctcagg cggggtgatg acctcagatt acagatggcc ctggaagaaa gccgaaggga
841cacagttaaa attccaaaaa agaaagagca tggctctctc ccacagcaga ctacgctgtt
901ggatttaatg gatgctctcc ccagctcggg ccccgcggcc cagaaagcag agccctgggg
961cccgtcagcc tccactaacc agaccaaccc ctggggcggg ccagcggctc ctgcgagtac
1021ttcagacccc tggccatcgt ttggtaccaa gccagctgcc tccattgacc catggggggt
1081gcccactgga gccaccgcac aatctgtccc caagaactcg gacccctggg cagcttcaca
1141gcagcctgcc tccagtgctg ggaaaagagc ttctgacgcg tggggcgcag tctccaccac
1201caagcccgtg tctgtctctg ggtcctttga gctcttcagt aatctgaatg gtacaattaa
1261agatgacttt tctgaatttg acaaccttcg gacttcaaaa aaaacagccg aatctgtgac
1321ctctctgcca tcccaaaaca atggaactac cagccctgac ccctttgagt ctcaacccct
1381gactgtcgcc tcaagcaagc ccagcagtgc ccggaaaaca cctgagtcct tcctgggccc
1441caacgcggcc ctggtgaacc tggactcact ggtgaccagg cctgccccac cagcccagtc
1501cctcaaccct ttcctggcac caggtgctcc cgccacctcg gcccctgtta accctttcca
1561ggtgaaccag ccccagccgc tgacactgaa ccagcttcgg gggagcccag tcctggggac
1621cagcacatcc tttgggcctg gcccaggagt ggagtccatg gctgtggcct cgatgacctc
1681cgcggcccca cagccagctc tgggggccac tggttcctct ctgacaccac tgggccctgc
1741aatgatgaac atggtgggca gtgtgggtat acccccatca gcagcccagg ccactggcac
1801aaccaaccct ttccttctct tgccaacttt cttgtacaaa gtggttggta agcctatccc
1861taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc ggtggcgcgt
1921taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.