Construct: ORF ccsbBroad304_14075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010401.1_s304c1, BRDN0000403860
- Derived from:
- ccsbBroadEn_14075
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDM1A (23028)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14075
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23028 | KDM1A | lysine demethylase 1A | NM_015013.4 | 85.6% | 85.4% | 1_366del;1214C>A;2370A>N |
| 2 | human | 23028 | KDM1A | lysine demethylase 1A | NM_001363654.2 | 85% | 84.8% | (many diffs) |
| 3 | human | 23028 | KDM1A | lysine demethylase 1A | XM_005245786.2 | 83.6% | 83.4% | (many diffs) |
| 4 | human | 23028 | KDM1A | lysine demethylase 1A | NM_001009999.3 | 83.2% | 83.1% | (many diffs) |
| 5 | human | 23028 | KDM1A | lysine demethylase 1A | XM_006710474.3 | 83% | 82.9% | (many diffs) |
| 6 | human | 23028 | KDM1A | lysine demethylase 1A | XM_017000717.1 | 78.7% | 77.6% | (many diffs) |
| 7 | human | 23028 | KDM1A | lysine demethylase 1A | XM_017000716.1 | 76.9% | 75.9% | (many diffs) |
| 8 | human | 23028 | KDM1A | lysine demethylase 1A | XM_006710473.3 | 76.3% | 75.3% | (many diffs) |
| 9 | mouse | 99982 | Kdm1a | lysine (K)-specific demethy... | NM_133872.2 | 77.9% | 85.2% | (many diffs) |
| 10 | mouse | 99982 | Kdm1a | lysine (K)-specific demethy... | XM_006539331.3 | 77.5% | 84.8% | (many diffs) |
| 11 | mouse | 99982 | Kdm1a | lysine (K)-specific demethy... | NM_001347221.1 | 76.2% | 83.2% | (many diffs) |
| 12 | mouse | 99982 | Kdm1a | lysine (K)-specific demethy... | XM_006539329.3 | 75.8% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2256
- ORF length:
- 2190
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tgaaagcttg gccaacctct cagaagatga gtattattca gaagaagaga 121 gaaatgccaa agcagagaag gaaaagaagc ttcccccacc accccctcaa gccccacctg 181 aggaagaaaa tgaaagtgag cctgaagaac catcgggtgt ggagggcgca gctttccaga 241 gccgacttcc tcatgaccgg atgacttctc aagaagcagc ctgttttcca gatattatca 301 gtggaccaca acagacccag aaggtttttc ttttcattag aaaccgcaca ctgcagttgt 361 ggttggataa tccaaagatt cagctgacat ttgaggctac tctccaacaa ttagaagcac 421 cttataacag tgatactgtg cttgtccacc gagttcacag ttatttagag cgtcatggtc 481 ttatcaactt cggcatctat aagaggataa aacccctacc aactaaaaag acaggaaagg 541 taattattat aggctctggg gtctcaggct tggcagcagc tcgacagtta caaagttttg 601 gaatggatgt cacacttttg gaagccaggg atcgtgtggg tggacgagtt gccacatttc 661 gcaaaggaaa ctatgtagct gatcttggag ccatggtggt aacaggtctt ggagggaatc 721 ctatggctgt ggtcagcaaa caagtaaata tggaactggc caagatcaag caaaaatgcc 781 cactttatga agccaacgga caagctgttc ctaaagagaa agatgaaatg gtagagcaag 841 agtttaaccg gttgctagaa gctacatctt accttagtca tcaactagac ttcaatgtcc 901 tcaataataa gcatgtgtcc cttggccagg cattggaagt tgtcattcag ttacaagaga 961 agcatgtcaa agatgagcag attgaacatt ggaagaagat agtgaaaact caggaagaat 1021 tgaaagaact tcttaataag atggtaaatt tgaaagagaa aattaaagaa ctccatcagc 1081 aatacaaaga agcatctgaa gtaaagccac ccagagatat tactgccgag ttcttagtga 1141 aaagcaaaca cagggatctg accgccctat gcaaggaata tgatgaatta gctgaaacac 1201 aaggaaagct agaagaaaaa cttcaggagt tggaagcgaa tcccccaagt gatgtatatc 1261 tctcatcaag agacagacaa atacttgatt ggcattttgc aaatcttgaa tttgctaatg 1321 ccacacctct ctcaactctc tcccttaagc actgggatca ggatgatgac tttgagttca 1381 ctggcagcca cctgacagta aggaatggct actcgtgtgt gcctgtggct ttagcagaag 1441 gcctagacat taaactgaat acagcagtgc gacaggttcg ctacacggct tcaggatgtg 1501 aagtgatagc tgtgaatacc cgctccacga gtcaaacctt tatttataaa tgcgacgcag 1561 ttctctgtac ccttcccctg ggtgtgctga agcagcagcc accagccgtt cagtttgtgc 1621 cacctctccc tgagtggaaa acatctgcag tccaaaggat gggatttggc aaccttaaca 1681 aggtggtgtt gtgttttgat cgggtgttct gggatccaag tgtcaatttg ttcgggcatg 1741 ttggcagtac gactgccagc aggggtgagc tcttcctctt ctggaacctc tataaagctc 1801 caatactgtt ggcactagtg gcaggagaag ctgctggtat catggaaaac ataagtgacg 1861 atgtgattgt tggccgatgc ctggccattc tcaaagggat ttttggtagc agtgcagtac 1921 ctcagcccaa agaaactgtg gtgtctcgtt ggcgtgctga tccctgggct cggggctctt 1981 attcctatgt tgctgcagga tcatctggaa atgactatga tttaatggct cagccaatca 2041 ctcctggccc ctcgattcca ggtgccccnc agccgattcc acgactcttc tttgcgggag 2101 aacatacgat ccgtaactac ccagccacag tgcatggtgc tctgctgagt gggctgcgag 2161 aagcgggaag aattgcagac cagtttttgg gggccatgta tacgctgcct cgccaggcca 2221 caccaggtgt tcctgcacag cagtccccaa gcatgtgccc aactttcttg tacaaagtgg 2281 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2341 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta