Construct: ORF ccsbBroad304_14113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003060.1_s304c1, BRDN0000400411
- Derived from:
- ccsbBroadEn_14113
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TPK1 (27010)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14113
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350885.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 2 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350886.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 3 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350887.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 4 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350889.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 5 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350893.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 6 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350894.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 7 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516047.2 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 8 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516048.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 9 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011982.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 10 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_024446717.1 | 99% | 4.3% | 14_16delAGAinsG;25A>G |
| 11 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350895.1 | 92.1% | 3.1% | 0_1ins31;2T>A |
| 12 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_024446716.1 | 92.1% | 3.1% | 0_1ins31;2T>A |
| 13 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011980.2 | 83.2% | 3.6% | 1_78del;92_94delAGAinsG;103A>G |
| 14 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011981.2 | 83.2% | 3.6% | 1_78del;92_94delAGAinsG;103A>G |
| 15 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350882.1 | 57% | 2.5% | 1_303del;317_319delAGAinsG;328A>G |
| 16 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350883.1 | 57% | 2.5% | 1_303del;317_319delAGAinsG;328A>G |
| 17 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350884.2 | 57% | 2.5% | 1_303del;317_319delAGAinsG;328A>G |
| 18 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350879.1 | 55.8% | 2.4% | 1_318del;332_334delAGAinsG;343A>G |
| 19 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_022445.4 | 55.8% | 2.4% | 1_318del;332_334delAGAinsG;343A>G |
| 20 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_005249970.1 | 55.8% | 2.4% | 1_318del;332_334delAGAinsG;343A>G |
| 21 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516039.2 | 55.8% | 2.4% | 1_318del;332_334delAGAinsG;343A>G |
| 22 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011974.1 | 55.8% | 2.4% | 1_318del;332_334delAGAinsG;343A>G |
| 23 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011975.1 | 55.8% | 2.4% | 1_318del;332_334delAGAinsG;343A>G |
| 24 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001042482.1 | 55.5% | 3.6% | (many diffs) |
| 25 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350880.1 | 55.5% | 3.6% | (many diffs) |
| 26 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516037.2 | 51.3% | 2.2% | 1_381del;395_397delAGAinsG;406A>G |
| 27 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011970.1 | 51.3% | 2.2% | 1_381del;395_397delAGAinsG;406A>G |
| 28 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011971.1 | 51.3% | 2.2% | 1_381del;395_397delAGAinsG;406A>G |
| 29 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516031.1 | 50.4% | 2.2% | 1_396del;410_412delAGAinsG;421A>G |
| 30 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516032.2 | 50.4% | 2.2% | 1_396del;410_412delAGAinsG;421A>G |
| 31 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516033.2 | 50.4% | 2.2% | 1_396del;410_412delAGAinsG;421A>G |
| 32 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516034.2 | 50.4% | 2.2% | 1_396del;410_412delAGAinsG;421A>G |
| 33 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350881.1 | 47.1% | 2.4% | (many diffs) |
| 34 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516035.3 | 42.7% | 2.2% | (many diffs) |
| 35 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011969.1 | 42.5% | 2.2% | (many diffs) |
| 36 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011972.1 | 40.4% | 2.4% | (many diffs) |
| 37 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516040.2 | 39.6% | 2.4% | (many diffs) |
| 38 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NR_146936.2 | 14.5% | (many diffs) | |
| 39 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | XM_006506219.3 | 53.5% | 1.8% | (many diffs) |
| 40 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | NM_013861.4 | 48.2% | 1.6% | (many diffs) |
| 41 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | XM_006506218.3 | 32% | .8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 102
- ORF length:
- 36
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ccaaaagagt agaagaagaa gacttaaagg ttgatgtgat cgtgacactg 121 ggaggccttg ctgggcgttt tgaccagatt atggcatctg tgaatacctt gttccaagcg 181 actcacatca ctccttttcc aattataata atccaagagg aatcgctgat ctacctgctc 241 caaccaggaa agcacaggtt gcatgtagac actggaatgg agggtgattg gtgtggcctt 301 attcctgttg gacagccttg tatgcaggtt acaaccacag gcctcaagtg gaacctcaca 361 aatgatgtgc ttgcttttgg aacattggtc agtacttcca atacctacga cgggtctggt 421 gttgtgactg tggaaactga ccacccactc ctctggacca tggccatcaa aagctaccca 481 actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct 541 acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt 601 a