Construct: ORF ccsbBroad304_14129

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011109.1_s304c1, BRDN0000406203
Derived from:
ccsbBroadEn_14129
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCAPER (49855)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14129
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 49855 SCAPER S-phase cyclin A associated... XM_011521656.3 37.1% 37% 1_1482del;2340C>A
2 human 49855 SCAPER S-phase cyclin A associated... XM_017022277.2 26% 26% 1_2481del;3339C>A
3 human 49855 SCAPER S-phase cyclin A associated... XM_017022278.2 26% 26% 1_2481del;3339C>A
4 human 49855 SCAPER S-phase cyclin A associated... XM_017022275.2 25.9% 25.8% 1_2499del;3357C>A
5 human 49855 SCAPER S-phase cyclin A associated... XM_017022276.1 25.9% 25.8% 1_2499del;3357C>A
6 human 49855 SCAPER S-phase cyclin A associated... NM_001145923.1 25.2% 25.2% 1_2586del;3444C>A
7 human 49855 SCAPER S-phase cyclin A associated... XM_024449939.1 25.2% 25.2% 1_2586del;3444C>A
8 human 49855 SCAPER S-phase cyclin A associated... XM_024449938.1 25.1% 25% 1_2604del;3462C>A
9 human 49855 SCAPER S-phase cyclin A associated... NM_001353010.2 23% 22.9% 1_2922del;3780C>A
10 human 49855 SCAPER S-phase cyclin A associated... NM_001353012.2 23% 22.9% 1_2922del;3780C>A
11 human 49855 SCAPER S-phase cyclin A associated... XM_017022273.1 23% 22.9% 1_2922del;3780C>A
12 human 49855 SCAPER S-phase cyclin A associated... NM_001353011.2 22.9% 22.8% 1_2940del;3798C>A
13 human 49855 SCAPER S-phase cyclin A associated... XM_011521653.3 22.9% 22.8% 1_2940del;3798C>A
14 human 49855 SCAPER S-phase cyclin A associated... NM_020843.4 20.8% 20.7% 1_3324del;4182C>A
15 human 49855 SCAPER S-phase cyclin A associated... XM_017022269.1 20.8% 20.7% 1_3324del;4182C>A
16 human 49855 SCAPER S-phase cyclin A associated... XM_017022270.1 20.8% 20.7% 1_3324del;4182C>A
17 human 49855 SCAPER S-phase cyclin A associated... NM_001353009.2 20.7% 20.6% 1_3342del;4200C>A
18 human 49855 SCAPER S-phase cyclin A associated... XM_005254417.2 20.7% 20.6% 1_3342del;4200C>A
19 human 49855 SCAPER S-phase cyclin A associated... XM_017022268.1 20.7% 20.6% 1_3342del;4200C>A
20 human 49855 SCAPER S-phase cyclin A associated... XM_017022272.1 20% 18.6% (many diffs)
21 human 49855 SCAPER S-phase cyclin A associated... XR_002957646.1 19.1% 1_3261del;4119C>A;4138_4577del
22 human 49855 SCAPER S-phase cyclin A associated... NR_148227.2 17% 1_3532del;4390C>A;4409_5140del
23 mouse 244891 Scaper S phase cyclin A-associated... XM_006511166.3 18.5% 18.1% (many diffs)
24 mouse 244891 Scaper S phase cyclin A-associated... NM_001081341.1 18.5% 18% (many diffs)
25 mouse 244891 Scaper S phase cyclin A-associated... XM_011242747.2 18.5% 18% (many diffs)
26 mouse 244891 Scaper S phase cyclin A-associated... XM_011242748.2 18.5% 18% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
942
ORF length:
876
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tctgattgac aaactgtgtg cctgcttcct ctcggtgcaa ggcccagtgg
121atgagaatcc caagatggcc atatttctgc agcatgccgc aggactctta catgcaatgt
181gtacactgtg ctttgctgtc actggaaggt catacagcat atttgacaat aatcgccagg
241atcccacagg gctgacagct gctcttcagg caaccgacct ggctggagtt cttcatatgc
301tctactgtgt cctcttccat ggcaccatct tggaccccag cactgccagt cccaaggaga
361attacactca aaataccatc caagtggcca ttcagagttt acgtttcttc aacagctttg
421cagctcttca tctgcctgct tttcagtcta ttgtaggggc agagggcttg tcccttgcat
481tccggcacat ggccagctcc ctgctgggcc actgcagcca agtctcctgt gaaagcctcc
541ttcatgaggt catcgtctgt gtgggctact tcactgtcaa ccacccagat aaccaggtga
601tcgtgcagtc cggccgccac cccacagtgc tgcagaagct ctgccagttg cccttccagt
661atttcagtga cccacggctg atcaaagtac tgttcccttc acttatcgct gcttgttaca
721acaaccatca gaacaagatc attctggagc aagagatgag ctgtgtttta ctggccactt
781tcattcagga tttggcacag actccaggtc aagcggaaaa ccagccttac caacccaaag
841ggaaatgcct tggttcccaa gactatcttg agctggctaa cagatttcct cagcaggcct
901gggaagaagc tcgacagttt ttattgaaaa aagagaaaaa atacccaact ttcttgtaca
961aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1021ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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