Construct: ORF ccsbBroad304_14130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014803.1_s304c1, BRDN0000400352
- Derived from:
- ccsbBroadEn_14130
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPLA8 (50640)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14130
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50640 | PNPLA8 | patatin like phospholipase ... | NM_001256007.3 | 99.9% | 99.4% | 2332delT |
| 2 | human | 50640 | PNPLA8 | patatin like phospholipase ... | NM_001256008.3 | 99.9% | 99.4% | 2332delT |
| 3 | human | 50640 | PNPLA8 | patatin like phospholipase ... | NM_015723.5 | 99.9% | 99.4% | 2332delT |
| 4 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XM_011516274.3 | 94.7% | 94.3% | (many diffs) |
| 5 | human | 50640 | PNPLA8 | patatin like phospholipase ... | NM_001256009.3 | 92% | 91.5% | 1692_1693ins186;2146delT |
| 6 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XM_005250396.5 | 92% | 91.5% | 1692_1693ins186;2146delT |
| 7 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XM_011516275.3 | 87.1% | 86.6% | (many diffs) |
| 8 | human | 50640 | PNPLA8 | patatin like phospholipase ... | NM_001256010.3 | 87.1% | 86.7% | 0_1ins300;2032delT |
| 9 | human | 50640 | PNPLA8 | patatin like phospholipase ... | NM_001256011.3 | 87.1% | 86.7% | 0_1ins300;2032delT |
| 10 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XM_017012261.2 | 79.2% | 78.7% | 0_1ins300;1392_1393ins186;1846delT |
| 11 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XR_001744795.2 | 64.5% | (many diffs) | |
| 12 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XR_001744793.2 | 57% | (many diffs) | |
| 13 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XR_001744797.2 | 56.7% | (many diffs) | |
| 14 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XR_001744796.2 | 56.1% | (many diffs) | |
| 15 | human | 50640 | PNPLA8 | patatin like phospholipase ... | XR_001744798.2 | 54.3% | (many diffs) | |
| 16 | mouse | 67452 | Pnpla8 | patatin-like phospholipase ... | NM_026164.2 | 84.5% | 81.8% | (many diffs) |
| 17 | mouse | 67452 | Pnpla8 | patatin-like phospholipase ... | XM_006516174.3 | 74.3% | 71.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2412
- ORF length:
- 2346
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tattaatctg actgtagata tatatattta cctccttagt aatgcaagaa 121 gtgtttgtgg gaagcagaga agcaagcaac tgtatttctt gttctcacct aagcattact 181 ggaggataag ccacatcagt ctacaaagag gttttcatac aaacataata agatgtaaat 241 ggaccaaaag tgaagcacat tcttgcagta agcactgtta ctctccaagc aaccatggtt 301 tacatattgg gattttgaaa cttagcactt ctgctcccaa gggacttaca aaagtgaaca 361 tttgtatgtc ccgtattaaa agtactttga actctgtttc aaaggctgtt tttggcaatc 421 aaaatgaaat gatttcacgt ttagctcaat ttaagccaag ttcccaaatt ttaagaaaag 481 tatcggatag tggctggtta aaacagaaaa acatcaaaca agccatcaaa tctctgaaaa 541 aatatagtga caaatcagca gaaaagagtc cttttccaga agagaaaagt cacattatag 601 acaaagaaga agatataggt aaacgcagtc tttttcatta cacaagttct ataaccacaa 661 aatttggaga ctcattctac tttttatcaa atcatattaa ttcatatttc aaacgtaagg 721 aaaaaatgtc tcaacaaaag gaaaatgaac atttccggga caaatcagaa cttgaagata 781 aaaaggtaga agaggggaaa ttaagatctc cagatcctgg catcctggct tataagccag 841 gctcagaatc tgtacatacg gtggacaagc ctacaagtcc ttctgcgata cctgatgttc 901 ttcaagtttc aactaaacaa agtattgcta actttctttc tcgtcccacg gaaggtgtac 961 aagctttagt aggtggttat attggtggac ttgtccccaa attaaagtat gattcaaaga 1021 gtcagtcaga agaacaggaa gagcctgcta aaactgatca ggctgtcagc aaagacagaa 1081 atgcagagga gaaaaagcgt ttatctcttc agcgagaaaa gattatcgca agggtgagta 1141 ttgataacag gacccgggca ttagttcagg cattaagaag aacaactgac ccaaagctct 1201 gcattactag ggttgaagaa ctgacttttc atcttctaga atttcctgaa ggaaaaggag 1261 tggctgtcaa ggaaagaatt attccatatt tattacgact gagacaaatt aaggatgaaa 1321 ctcttcaggc tgcagttaga gaaattttgg ccctaattgg ctatgtggat ccagtgaaag 1381 ggagaggaat ccgaattctc tcaattgatg gtggaggaac aaggggcgtg gttgctctcc 1441 agaccctacg aaaattagtt gaacttactc agaagccagt tcatcagctc tttgattaca 1501 tttgtggtgt aagcacaggt gccatattag ctttcatgtt ggggttgttt catatgccct 1561 tggatgaatg tgaggaactt tatcgaaaat taggatcaga tgtattttca caaaatgtca 1621 ttgttggaac agtaaaaatg agttggagcc atgcatttta tgacagtcaa acatgggaaa 1681 acattcttaa ggataggatg ggatctgcac tgatgattga aacagcaaga aaccccacat 1741 gtcctaaggt agctgctgta agtaccatag taaatagagg gataacaccc aaagcttttg 1801 tgttcagaaa ctatggtcat tttcctggaa tcaactctca ttatttggga ggctgtcagt 1861 ataaaatgtg gcaggccatt agagcctcat ctgctgctcc aggctacttt gcagaatatg 1921 cattgggaaa tgatcttcat caagatggag gtttgcttct gaataaccct tcggcattag 1981 ctatgcatga gtgtaaatgt ctttggccag atgtgccgtt agagtgcata gtatccctgg 2041 gcactggacg ttatgagagt gatgtgagaa acacggtaac atacacaagc ttgaaaacta 2101 aactttctaa tgttatcaac agtgctacag atacagaaga agtccatata atgcttgatg 2161 gcctgttacc tcctgacacc tattttagat tcaatcctgt aatgtgtgaa aacatacctc 2221 tagatgaaag tcgaaatgaa aagctggatc agctgcagtt ggaagggttg aaatacatag 2281 aaagaaatga acaaaaaatg aaaaaagttg caaaaatatt aagtcaagaa aaaacaactc 2341 tgcagaaaat taatgattgg ataaaattaa aaactgatat gtatgaagga cttccatctt 2401 ttcaaaattg tgcccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct 2461 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac 2521 aatcaacctc tggatta