Transcript: Human NM_001256007.3

Homo sapiens patatin like phospholipase domain containing 8 (PNPLA8), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-28
Taxon:
Homo sapiens (human)
Gene:
PNPLA8 (50640)
Length:
4569
CDS:
237..2585

Additional Resources:

NCBI RefSeq record:
NM_001256007.3
NBCI Gene record:
PNPLA8 (50640)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256007.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365718 GGCATTAGTTCAGGCATTAAG pLKO_005 1328 CDS 100% 13.200 18.480 N PNPLA8 n/a
2 TRCN0000055816 CCATAGTAAATAGAGGGATAA pLKO.1 1936 CDS 100% 10.800 15.120 N PNPLA8 n/a
3 TRCN0000055813 CGAACAAGAAACCGAAAGCTA pLKO.1 2895 3UTR 100% 3.000 4.200 N PNPLA8 n/a
4 TRCN0000370939 ACGGAAGGTGTACAAGCTTTA pLKO_005 1119 CDS 100% 10.800 8.640 N PNPLA8 n/a
5 TRCN0000055814 CGGGCATTAGTTCAGGCATTA pLKO.1 1326 CDS 100% 10.800 8.640 N PNPLA8 n/a
6 TRCN0000365717 CATTTGTATGTCCCGTATTAA pLKO_005 530 CDS 100% 15.000 10.500 N PNPLA8 n/a
7 TRCN0000055815 CCAGGCTACTTTGCAGAATAT pLKO.1 2070 CDS 100% 13.200 9.240 N PNPLA8 n/a
8 TRCN0000377644 CCTTACTGTGCTAGTAGATTT pLKO_005 2819 3UTR 100% 13.200 9.240 N PNPLA8 n/a
9 TRCN0000370998 GCCGTTAGAGTGCATAGTATC pLKO_005 2186 CDS 100% 10.800 7.560 N PNPLA8 n/a
10 TRCN0000376669 TTATCGCAAGGGTGAGTATTG pLKO_005 1294 CDS 100% 10.800 7.560 N PNPLA8 n/a
11 TRCN0000055817 CCTAAGCATTACTGGAGGATA pLKO.1 339 CDS 100% 4.950 3.465 N PNPLA8 n/a
12 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 3491 3UTR 100% 4.950 2.475 Y CFLAR n/a
13 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 3491 3UTR 100% 4.950 2.475 Y C19orf31 n/a
14 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 3489 3UTR 100% 4.950 2.475 Y ERN2 n/a
15 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 3489 3UTR 100% 4.950 2.475 Y P3H4 n/a
16 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 3489 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256007.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14130 pDONR223 100% 99.9% 99.4% None 2332delT n/a
2 ccsbBroad304_14130 pLX_304 0% 99.9% 99.4% V5 (not translated due to frame shift) 2332delT n/a
3 TRCN0000466476 AGGGGTTTGCTCTTCAAAACCCAT pLX_317 17.6% 99.9% 99.4% V5 (not translated due to frame shift) 2332delT n/a
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