Construct: ORF ccsbBroad304_14150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018066.1_s304c1, BRDN0000408356
- Derived from:
- ccsbBroadEn_14150
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SRRT (51593)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14150
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51593 | SRRT | serrate, RNA effector molecule | NM_001128854.2 | 99.9% | 12.6% | 303_304insG |
| 2 | human | 51593 | SRRT | serrate, RNA effector molecule | NM_001128853.2 | 99.8% | 12.6% | 303_304insG;1159_1161delGAA |
| 3 | human | 51593 | SRRT | serrate, RNA effector molecule | NM_001128852.2 | 99.5% | 12.5% | 303_304insG;2324_2335del |
| 4 | human | 51593 | SRRT | serrate, RNA effector molecule | NM_015908.6 | 99.3% | 12.5% | 303_304insG;1159_1161delGAA;2327_2338del |
| 5 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_005250408.2 | 99.1% | 12.5% | 1_21del;324_325insG |
| 6 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_005250407.2 | 99% | 12.5% | 1_21del;324_325insG;1180_1182delGAA |
| 7 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_005250406.2 | 98.7% | 12.4% | 1_21del;324_325insG;2345_2356del |
| 8 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_005250405.2 | 98.6% | 12.4% | (many diffs) |
| 9 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_024446793.1 | 96.1% | 12.1% | 1_21del;324_325insG;1061_1144del |
| 10 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_017012291.1 | 90.8% | 3.5% | 0_1ins237;66_67insG |
| 11 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_017012290.1 | 90.7% | 3.5% | 0_1ins237;66_67insG;922_924delGAA |
| 12 | human | 51593 | SRRT | serrate, RNA effector molecule | XM_024446794.1 | 73.9% | 3.5% | 0_1ins682 |
| 13 | mouse | 83701 | Srrt | serrate RNA effector molecu... | XM_006504629.1 | 88.3% | 12.5% | (many diffs) |
| 14 | mouse | 83701 | Srrt | serrate RNA effector molecu... | NM_031405.2 | 87.9% | 12.4% | (many diffs) |
| 15 | mouse | 83701 | Srrt | serrate RNA effector molecu... | NM_001109910.1 | 87.5% | 12.6% | (many diffs) |
| 16 | mouse | 83701 | Srrt | serrate RNA effector molecu... | NM_001109909.1 | 87.1% | 12.5% | (many diffs) |
| 17 | mouse | 83701 | Srrt | serrate RNA effector molecu... | XM_006504630.1 | 78% | 1.4% | (many diffs) |
| 18 | mouse | 83701 | Srrt | serrate RNA effector molecu... | XM_006504631.1 | 70.2% | 3.7% | (many diffs) |
| 19 | mouse | 83701 | Srrt | serrate RNA effector molecu... | XM_006504632.1 | 64.5% | 4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtgacagt gatgacgagt acgatcgaag gcgcagggac aagttcagaa 121 gagagcgcag cgactacgac cgttcccgcg agagagatga aagacgtcga ggggacgatt 181 ggaatgacag agagtgggac cgtggccgtg agcgccgtag tcggggtgaa tatcgggact 241 atgaccggaa tcggcgagag cgcttctcgc cacctcgcca cgaactcagc ccgccacaga 301 agcgcatgag gagagactgg gatgagcaca gctctgaccc ataccacagt ggctatgaga 361 tgccctatgc tggggggggg tgggggccca acttatggcc cccctcagcc ctggggccac 421 cctgacgtcc acatcatgca gcaccatgtc ctgcctatcc aggccaggct gggcagcatt 481 gcagagattg acctgggtgt gccgccgccc gtgatgaaga ccttcaagga gtttctcctc 541 tccctggatg actcggtgga tgagacggag gccgtcaagc gctataatga ctacaagctg 601 gatttccgga ggcaacagat gcaggatttc ttcctggcgc acaaagatga ggagtggttt 661 cggtctaagt accacccaga tgaggtgggg aagcgtcggc aggaggcccg gggggccctg 721 caaaaccgac tgagggtctt cctgtccctc atggagactg gctggtttga taaccttctc 781 ctggacatag acaaagctga tgccattgtc aagatgctgg atgcagccgt gattaagatg 841 gaaggaggca cggagaatga tcttcgcatc ctggagcagg aggaggagga ggagcaggca 901 ggaaagcctg gggagcccag caagaaagaa gaaggacggg ctggagcagg cctaggggac 961 ggggagcgca aaaccaacga caaggatgag aagaaggaag acggcaagca ggctgagaat 1021 gacagttcta atgatgacaa aacaaagaag tcggagggtg atggggacaa ggaagagaag 1081 aaagaagact ccgagaagga agccaaaaag agtagcaaga agcggaaccg gaagcacagt 1141 ggtgacgaca gctttgacga gggcagcgtg tcagagtctg agtcggagtc agagagcggc 1201 caggctgagg aggagaagga ggaggccgaa gcgctcaagg agaaggagaa gcccaaggaa 1261 gaagaatggg agaagcccaa ggacgccgcg gggctggagt gcaagccgcg gccgctgcat 1321 aagacctgct ccctcttcat gcgcaacatc gcgcccaaca tctcccgggc cgagatcatc 1381 tccctttgta aaaggtaccc aggctttatg cgggtggcgc tctcagagcc ccagccagag 1441 aggaggtttt tccgtcgtgg ctgggtgacc ttcgaccgca gtgttaacat taaagagatc 1501 tgttggaacc tgcagaacat ccgtctccgg gagtgtgagc tgagccctgg tgtgaacagg 1561 gacctgaccc ggcgcgttcg caacatcaac ggcatcaccc agcacaagca gattgtgcgc 1621 aacgacatca agctggcggc caagctgatc cacacgctgg atgacaggac acagctttgg 1681 gcctcagaac cagggacgcc tcccctgccc acgagcctgc cctcgcaaaa cccgatcttg 1741 aagaatatca ccgactacct gatcgaggaa gtaagcgccg aggaggagga gctgctgggg 1801 agcagcgggg gcgctcctcc tgaggagcct cctaaggaag ggaacccggc agagatcaac 1861 gtggagcggg atgagaagtt gattaaggtc ttggacaagc tcctccttta cctgcgcatc 1921 gtgcattcct tggattatta caacacctgt gagtacccca acgaggacga gatgcccaat 1981 cgctgtggga tcatccacgt tcgggggccc atgccaccca accgcatcag tcacggggaa 2041 gtgctggagt ggcagaagac ttttgaggag aagctcacgc cgttgctgag tgtgcgggag 2101 tcactctcag aggaagaggc ccagaagatg gggcgcaaag acccagagca ggaagtggag 2161 aagttcgtca cctccaacac gcaggaactg ggcaaggata agtggctgtg tcctctcagt 2221 ggcaagaaat tcaagggtcc tgagtttgtg cgcaaacata tcttcaacaa gcatgcagag 2281 aaaattgagg aagtgaaaaa ggaagtcgcg ttttttaaca acttcctcac tgatgctaag 2341 cgcccagctc tgcctgagat caagccagcc cagccacctg gccccgccca gagtttgacc 2401 ccaggactcc cctacccaca ccagactccc cagggcctga tgccctatgg tcagccccgg 2461 cccccgatct tgggctatgg agctggtgct gtccgccctg cagtccccac aggaggccct 2521 ccataccccc atgccccgta tggtgctggt cgagggaact atgatgcctt ccgaggccag 2581 ggaggttatc ctgggaaacc tcgcaacagg atggttcgtg gagacccaag ggccattgtg 2641 gaatatcggg acctggatgc cccagacgat gttgatttct ttttgccaac tttcttgtac 2701 aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga 2761 gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta