Construct: ORF ccsbBroad304_14164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013495.1_s304c1, BRDN0000400240
- Derived from:
- ccsbBroadEn_14164
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT1A10 (54575)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14164
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54575 | UGT1A10 | UDP glucuronosyltransferase... | NM_019075.2 | 99.8% | 99.2% | 4G>A;1580delC;1582A>G |
| 2 | human | 54576 | UGT1A8 | UDP glucuronosyltransferase... | NM_019076.4 | 96.6% | 93.7% | (many diffs) |
| 3 | human | 54577 | UGT1A7 | UDP glucuronosyltransferase... | NM_019077.2 | 95.7% | 93% | (many diffs) |
| 4 | human | 54600 | UGT1A9 | UDP glucuronosyltransferase... | NM_021027.3 | 95.5% | 92.4% | (many diffs) |
| 5 | human | 54578 | UGT1A6 | UDP glucuronosyltransferase... | NM_205862.2 | 49.2% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tcgcgcaggg tggaccagcc ccgttccttt atgtgtgtgt ctactgctga 121 cctgtggctt tgccgaggca gggaagctgc tggtagtgcc catggatggg agtcactggt 181 tcaccatgca gtcggtggtg gagaaactta tcctcagggg gcatgaggtg gttgtagtca 241 tgccagaggt gagttggcaa ctggaaagat cactgaattg cacagtgaag acttactcaa 301 cctcgtacac tctggaagat cagaaccggg aattcatggt tttcgcccat gctcaatgga 361 aagcacaggc acaaagtata ttttctctat taatgagttc atccagtggt tttcttgact 421 tatttttttc gcattgcagg agtttgttta atgaccgaaa attagtagaa tacttaaagg 481 agagttcttt tgatgcagtg tttctggatc cttttgatac ctgtggctta attgttgcta 541 aatatttctc cctcccctct gtggtcttca ccaggggaat attttgccac catcttgaag 601 aaggtgcaca gtgccctgct cctctttcct atgtccccaa tgatctctta gggttctcag 661 atgccatgac tttcaaggag agagtatgga accacatcgt gcacttggag gaccatttat 721 tttgccagta tctttttaga aatgccctag aaatagcctc tgaaattctc caaacccctg 781 tcacggcata tgatctctac agtcacacat caatttggtt gttgcgaacg gactttgttt 841 tggactatcc caaacccgtg atgcccaaca tgatcttcat tggtggtatc aactgtcatc 901 agggaaagcc attgcctatg gaatttgaag cctacattaa tgcttctgga gaacatggaa 961 ttgtggtttt ctctttggga tcaatggtct cagaaattcc agagaagaaa gctatggcaa 1021 ttgctgatgc tttgggcaaa atccctcaga cagtcctgtg gcggtacact ggaacccgac 1081 catcgaatct tgcgaacaac acgatacttg ttaagtggct accccaaaac gatctgcttg 1141 gtcacccgat gacccgtgcc tttatcaccc atgctggttc ccatggtgtt tatgaaagca 1201 tatgcaatgg cgttcccatg gtgatgatgc ccttgtttgg tgatcagatg gacaatgcaa 1261 agcgcatgga gactaaggga gctggagtga ccctgaatgt tctggaaatg acttctgaag 1321 atttagaaaa tgctctaaaa gcagtcatca atgacaaaag ttacaaggag aacatcatgc 1381 gcctctccag ccttcacaag gaccgcccgg tggagccgct ggacctggcc gtgttctggg 1441 tggagtttgt gatgaggcac aagggcgcgc cacacctgcg ccccgcagcc cacgacctca 1501 cctggtacca gtaccattcc ttggacgtga ttggtttcct cttggccgtc gtgctgacag 1561 tggccttcat cacctttaaa tgttgtgctt atggctaccg gaaatgcttg gggaaaaaag 1621 ggcgagttaa gaaagcccac aaatcgagac ccattgccca actttcttgt acaaagtggt 1681 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc 1741 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a