Construct: ORF ccsbBroad304_14190

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007310.1_s304c1, BRDN0000409535
Derived from:
ccsbBroadEn_14190
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
P3H2 (55214)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14190
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55214 P3H2 prolyl 3-hydroxylase 2 NM_001134418.1 99.8% 99.2% 153C>T;1566delC
2 human 55214 P3H2 prolyl 3-hydroxylase 2 XM_011512955.1 99.8% 99.2% 153C>T;1566delC
3 human 55214 P3H2 prolyl 3-hydroxylase 2 NM_018192.4 74.3% 73.8% 1_543del;696C>T;2109delC
4 mouse 210530 P3h2 prolyl 3-hydroxylase 2 NM_173379.2 64.1% 68.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1647
ORF length:
1581
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga aatgcagcag aacattgaga attacagggc gacagctggt gttgaagcat
121tgcagttggt agacagagaa gccaagccac acatggagag ttacaatgca ggagttaaac
181attatgaggc tgatgacttt gagatggcta tcaggcattt cgaacaagcc ttaagagaat
241atttcgttga agatacagaa tgccggaccc tatgtgaggg gcctcagaga tttgaagaat
301atgagtattt agggtataag gctggtctgt atgaagctat tgcagatcac tacatgcagg
361tgcttgtttg tcagcatgaa tgtgtgaggg aacttgccac ccgccctggc cgcctctctc
421ccatcgagaa ttttcttcct ctgcactatg attacctaca gtttgcctac tatcgagttg
481gtgagtatgt gaaagccctg gagtgtgcca aagcctatct tctatgccat ccagatgatg
541aggatgtcct agacaatgtg gattactatg agagtctgct ggatgatagc attgacccgg
601catccattga ggccagagag gatttaacaa tgtttgtgaa acgtcataag ctggagtctg
661agctgataaa atcagctgca gaaggtctgg ggttttcata cactgaaccg aattattgga
721tcagatatgg aggacgacag gatgagaatc gggtcccttc aggagtgaac gtagagggag
781cagaagttca tggattctca atgggaaaaa agctatcacc caagatagat cgagacctaa
841gagaaggtgg tcctctactc tatgagaaca tcacattcgt ctacaactcg gagcagctga
901acgggactca gcgggttctc ctggataacg tcctgtcgga agaacagtgc cgggagctcc
961acagcgtggc cagtggaatc atgcttgttg gtgatggata cagaggaaaa acttcacccc
1021atacacccaa tgaaaagttt gaaggtgcaa ctgtcctgaa agcactcaaa tctggttatg
1081aaggtcgagt cccactgaag agcgctcgtc tgttttatga catcagcgaa aaggctcgaa
1141ggattgtaga atcttatttt atgctgaact caactctgta tttttcctat acacacatgg
1201tctgccgaac agccctgtct ggtcagcagg atagaagaaa tgacctcagt catcccatcc
1261atgctgacaa ctgtttgttg gatccagagg ccaacgaatg ctggaaggag cctcctgctt
1321acacatttcg agactatagt gctctcctat atatgaatga tgactttgaa ggaggagaat
1381tcatattcac agagatggat gctaagactg tgactgcctc tataaaacca aaatgtgggc
1441gcatgatcag cttctcatct ggaggagaga accctcatgg ggtgaaggca gtcaccaagg
1501gaaagaggtg tgctgtggct ctgtggttca ccttggaccc actttataga gaattggagc
1561gaatacaggc tgatgaagtg attgcaattc tggatcaaga acagcaaggg aagcatgaac
1621tgaatatcaa cctaaagatg agctataccc aactttcttg tacaaagtgg ttggtaagcc
1681tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg
1741gcgcgttaag tcgacaatca acctctggat ta

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